Differential expression of cyclic nucleotide phosphodiesterases 4 in developing rat lung

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Emmanuel LopezCéline Méhats

Abstract

During the perinatal period, lungs undergo changes to adapt to air breathing. The genes involved in these changes are developmentally regulated by various signaling pathways, including the cyclic nucleotide cAMP. As PDE4s are critical enzymes for regulation of cAMP levels, the objective of this study was to investigate PDE4's ontogeny in developing rat lung during the perinatal period. Pulmonary PDE4 activity, PDE4A-D, PDE4B, and PDE4D variant expression levels, PDE4B and PDE4D protein levels, and PDE4D localization in distal lung were determined. PDE4 activity increased towards term, dropped at birth, and increased thereafter to reach a plateau at the end of the second week of life. PDE4B2 and PDE4D long forms demonstrated a pattern of expression that increased markedly at birth. After birth, PDE4D was expressed in alveolar epithelial and mesenchymal cells. The study, therefore, evidenced striking variations in expression patterns among the PDE4 family that differed from changes in global PDE4 activity.

References

Jan 1, 1985·Experimental Lung Research·J A WhitsettJ Lessard
Jan 1, 1974·Methods in Enzymology·W J ThompsonM M Appleman
Jan 11, 1982·Life Sciences·J A WhitsettJ A Burdsall
Dec 1, 1983·Pediatric Research·J A WhitsettC Darovec-Beckerman
Apr 16, 1998·The Journal of Clinical Investigation·N FinleyH G Folkesson
Jun 2, 1998·Biochimica Et Biophysica Acta·M J AcarreguiS T Penisten
Feb 22, 2001·Annual Review of Physiology·W V Cardoso
Dec 26, 2001·Trends in Endocrinology and Metabolism : TEM·Celine MehatsMarco Conti
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 21, 2002·The Journal of Biological Chemistry·Marco ContiCatherine Jin
Oct 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Céline MéhatsMarco Conti
Nov 11, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Linda Biadasz ClerchDonald Massaro
Aug 8, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jennifer J FosterJeanne M Snyder
Sep 14, 2006·Pharmacological Reviews·Andrew T Bender, Joseph A Beavo
Oct 4, 2007·Physiological Genomics·Olivier BoucheratJacques R Bourbon
Jul 29, 2008·British Journal of Pharmacology·D Spina

❮ Previous
Next ❯

Citations

Oct 24, 2012·Pharmacology & Therapeutics·Bart G J DekkersMartina Schmidt
Nov 14, 2013·Biochemical and Biophysical Research Communications·Zhijun HuangQiong Wu
Jul 26, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Issaka YougbareEric Rousseau

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.