PMID: 3383854Jun 1, 1988Paper

Differential expression of four linked sheep metallothionein genes

European Journal of Biochemistry
M G Peterson, J F Mercer

Abstract

Regulation of the closely linked endogenous sheep MT-Ia, MT-Ib, MT-Ic and MT-II genes by heavy metals and dexamethasone was studied in cultured sheep fibroblasts. Only MT-II mRNA was detectable before addition of any inducer. Addition of copper, zinc or cadmium salts to the culture medium increased the level of each mRNA; however, the magnitude of this response varied greatly between the four metallothionein genes. Following induction, levels of MT-Ia mRNA were the highest, followed by MT-II and MT-Ic mRNAs. The MT-Ib mRNA was only present at low levels. Zinc and cadmium were found to be the most effective inducers of each gene. The maximal response of the sMT-Ia, Ib and II genes to copper was only 30% of zinc and cadmium. The sMT-Ic gene responded very weakly to copper, less than 5% of the levels achieved with zinc. Only the MT-II mRNA increased in response to dexamethasone. In the liver of sheep on normal diets, the levels of MT-Ia, Ic and II mRNAs were found to be unexpectedly high and comparable to induced levels in fibroblasts.

References

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Nov 3, 1986·European Journal of Biochemistry·M G Peterson, J F Mercer
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Citations

Jul 1, 1995·Neurochemistry International·M EbadiR F Pfeiffer
Nov 1, 1994·General Pharmacology·M Ebadi, P L Iversen
Feb 1, 1996·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·J M BouquegneauS Havelange
Apr 1, 1997·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A F HollowayA K West
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G MorahanJ F Miller
May 31, 1990·European Journal of Biochemistry·J A PaynterJ F Mercer
Jul 1, 1995·The Proceedings of the Nutrition Society·I Bremner, J H Beattie
Apr 8, 2000·Critical Reviews in Biochemistry and Molecular Biology·A T MilesV Rodilla
Jul 12, 2016·Metallomics : Integrated Biometal Science·Julian F B Mercer, James Camakaris

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