Differential Expression of Gene Profiles in MRGX-treated Lung Cancer

Journal of Pharmacopuncture
Yong-Kyun KwonIk-Soon Jang

Abstract

Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles. To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis, we grouped the genes into up-and down-regulated profiles and into ontology-related regulated genes and proteins through their interaction network. One hundred nine proteins that were up- and down-regulated by MRGX were queried by using IPA. IL8, MMP7 and PLAUR and were found to play a major role in the anti-cancer activity in MRGX-treated lung cancer cells. These results were validated using a Western blot analysis and a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. Most MRGX-responsive genes are up-regulated transiently in A549 cells, but down-regulated in a sustained manner in lung cancer cells.

References

Dec 19, 2001·Journal of Korean Medical Science·T K YunH Y Yun
Nov 3, 2007·American Journal of Respiratory and Critical Care Medicine·I-Ming WangPeter D Paré
May 29, 2009·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Oct 23, 2009·Journal of Agricultural and Food Chemistry·Do Yeon KimSung Hyun Chung
Feb 3, 2012·The American Journal of Chinese Medicine·Jeong Won HwangJong-Soon Choi
May 29, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hye-Min ParkHyung-Sub Kang

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Methods Mentioned

BETA
chip
electrophoresis
PCR
X-ray

Software Mentioned

IPA
Ingenuity Pathways

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