Differential expression of genes encoding CD30L and P-selectin in cattle with Johne's disease: progress toward a diagnostic gene expression signature

Veterinary Immunology and Immunopathology
Kerstin SkovgaardPaul M Coussens

Abstract

Mycobacterium avium subspecies paratuberculosis (Mycobacterium paratuberculosis), the causative agent of paratuberculosis (paraTB) or Johne's disease in ruminants, is a health problem for the global cattle industry with significant economic losses related to decreased milk production and reduced fertility. Commonly paraTB in cattle is diagnosed by antibody detection by serum enzyme-linked immunosorbent assay (ELISA), by detection of the pathogen by cultivation of individual faecal samples, or by in vitro measurement of cell mediated immune responses using the IFN-gamma test. There is an ongoing need for developing new diagnostic approaches as all currently available diagnostic tests for paraTB may fail to detect sub-clinical infection. We used cDNA microarrays to simultaneously measure expression of over 1300 host genes to help identify a subset of gene expression changes that might provide a unique gene expression signature for paraTB infection. In the present study, non-stimulated leukocytes isolated from 10 sub-clinical paraTB infected cows were examined for genes being expressed at significantly different levels than in similar cells from control cows with the same herd background. We included cattle (Holstein) from two loc...Continue Reading

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Citations

Jan 5, 2011·Clinical & Developmental Immunology·W Ray WatersD Mark Estes
Nov 5, 2010·Veterinary Immunology and Immunopathology·John P Bannantine, Adel M Talaat
Nov 15, 2013·Veterinary Pathology·G HostetterS D Jewell
Dec 13, 2007·Veterinary Research·Shivani Ojha, Magdalena Kostrzynska
Jun 3, 2009·Transboundary and Emerging Diseases·D E MacHughK G Meade

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