Differential Expression of Genes Involved in Host Recognition, Attachment, and Degradation in the Mycoparasite Tolypocladium ophioglossoides

G3 : Genes - Genomes - Genetics
C Alisha QuandtJoseph W Spatafora

Abstract

The ability of a fungus to infect novel hosts is dependent on changes in gene content, expression, or regulation. Examining gene expression under simulated host conditions can explore which genes may contribute to host jumping. Insect pathogenesis is the inferred ancestral character state for species of Tolypocladium, however several species are parasites of truffles, including Tolypocladium ophioglossoides. To identify potentially crucial genes in this interkingdom host switch, T. ophioglossoides was grown on four media conditions: media containing the inner and outer portions of its natural host (truffles of Elaphomyces), cuticles from an ancestral host (beetle), and a rich medium (Yeast Malt). Through high-throughput RNASeq of mRNA from these conditions, many differentially expressed genes were identified in the experiment. These included PTH11-related G-protein-coupled receptors (GPCRs) hypothesized to be involved in host recognition, and also found to be upregulated in insect pathogens. A divergent chitinase with a signal peptide was also found to be highly upregulated on media containing truffle tissue, suggesting an exogenous degradative activity in the presence of the truffle host. The adhesin gene, Mad1, was highly exp...Continue Reading

References

Nov 5, 1997·Current Opinion in Structural Biology·B Henrissat, G Davies
Sep 18, 1999·Current Opinion in Microbiology·B CormackS M Beverley
Aug 11, 2001·Biochemical Society Transactions·J K Chugh, B A Wallace
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John D Storey, Robert Tibshirani
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Sep 15, 2007·Molecular Microbiology·Jean-Paul Latgé
May 21, 2008·Studies in Mycology·Gi-Ho SungJoseph W Spatafora
May 22, 2009·Nature·Daniel M RosenbaumBrian K Kobilka
Nov 7, 2009·Nucleic Acids Research·Gabriel OstlundErik L L Sonnhammer
May 5, 2010·Genetics·Paul L Auer, R W Doerge
May 13, 2010·Applied Microbiology and Biotechnology·André Schuster, Monika Schmoll
May 21, 2010·Cellular Microbiology·Jean-Paul Latgé
Jul 1, 2001·Molecular Plant Pathology·A Idnurm, B J Howlett
Oct 15, 2010·Fungal Biology·Hongxia ZhengQihan Wu
Dec 15, 2010·Bioinformatics·Michael E SmootTrey Ideker
May 3, 2011·Applied and Environmental Microbiology·Barbara ReithnerAlfredo Herrera-Estrella
Aug 23, 2011·Applied and Environmental Microbiology·Sabine GruberVerena Seidl-Seiboth
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Nov 1, 2011·PLoS Computational Biology·Sean R Eddy
Jan 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bing WangChengshu Wang
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
May 31, 2012·Nucleic Acids Research·Yanbin YinYing Xu
Mar 19, 2013·Statistical Applications in Genetics and Molecular Biology·Yanming DiJeff H Chang

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Citations

Dec 29, 2020·The New Phytologist·Lotus A LofgrenPeter G Kennedy
Apr 26, 2017·Current Opinion in Microbiology·Ryan M KeplerStephen A Rehner
Mar 11, 2017·Microbiology Spectrum·Magnus KarlssonSusanne Zeilinger

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Datasets Mentioned

BETA
SRP062415
SRR2179765

Methods Mentioned

BETA
reverse transcription PCR
RNA-Seq

Software Mentioned

Bowtie
RAxML
Short Read Toolbox
Hmmer
perl
gplots
R
NBPSeq
BiNGO
TMHMM

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