PMID: 11316565Apr 24, 2001Paper

Differential expression of growth factors in irradiated mouse testes

International Journal of Radiation Oncology, Biology, Physics
Claire MauduitMohamed Benahmed

Abstract

By using as an experimental model the male mouse gonad, which contains both radiosensitive (germ) and radioresistant (somatic) cells, we have studied the growth factor (and/or receptor) expression of transforming growth factor-beta receptor (TGFbeta RI), stem cell factor (SCF), c-kit, Fas-L, Fas, tumor necrosis factor receptor (TNF R55), and leukemia inhibiting factor receptor (LIF-R) after local irradiation. Adult male mice were locally irradiated on the testes. Induction of apoptosis in the different testicular cell types following X-ray radiation was identified by the TdT-mediated dUTP Nick End Labeling (TUNEL) approach. Growth factor expression was evidenced by semiquantitative RT-PCR and Western blot analyses. Apoptosis, identified through the TUNEL approach, occurred in radiosensitive testicular (premeotic) germ cells with the following kinetics: the number of apoptotic cells increased after 24 h (p < 0.001) and was maximal 48 h after a 2-Gy ionizing radiation (p < 0.001). Apoptotic cells were no longer observed 72 h after a 2-Gy irradiation. The number of apoptotic cells increased with the dose of irradiation (1-4 Gy). In the seminiferous tubules, the growth factor expression in premeiotic radiosensitive germ cells was m...Continue Reading

References

Jan 1, 1975·Current Topics in Molecular Endocrinology·V HanssonS N Nayfeh
May 1, 1975·Molecular and Cellular Endocrinology·L HagenäsS N Nayfeh
Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Apr 30, 1991·Biochemical and Biophysical Research Communications·P RossiR Geremia
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D E HallahanR R Weichselbaum
Feb 9, 1995·The New England Journal of Medicine·A S Lichter, T S Lawrence
Jun 1, 1993·Biology of Reproduction·L A CookerE Goldberg
Dec 17, 1998·Zygote : the Biology of Gametes and Early Embryos·G Durcova-HillsG Brem
Apr 15, 1999·International Journal of Radiation Biology·N GuittonC Chenal
Aug 24, 1999·Pharmacology & Therapeutics·J R Woodburn

❮ Previous
Next ❯

Citations

May 7, 2010·Journal of Molecular Histology·Mehmet KanterSule Parlar
Jul 24, 2013·Journal of Assisted Reproduction and Genetics·Mohammadreza GholamiJavad Mohammadi-Asl
Feb 14, 2009·Pharmacogenetics and Genomics·Markus A SchirmerJürgen Brockmöller
Jun 26, 2009·Reproductive Biology and Endocrinology : RB&E·Imen HammamiClaire Mauduit
Oct 25, 2013·International Journal of Biological Sciences·Liren QianJianming Cai
Sep 25, 2012·Basic & Clinical Pharmacology & Toxicology·Zhitao JiangDapeng Jiang
Jun 20, 2007·International Journal of Radiation Biology·Sonia ChaterClaire Mauduit
Apr 12, 2003·Melanoma Research·Frédéric MouriauxSimon Saule
Oct 26, 2021·International Journal of Radiation Biology·Ahmed I SemaidaShady A Elfouly

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis