Abstract
By using as an experimental model the male mouse gonad, which contains both radiosensitive (germ) and radioresistant (somatic) cells, we have studied the growth factor (and/or receptor) expression of transforming growth factor-beta receptor (TGFbeta RI), stem cell factor (SCF), c-kit, Fas-L, Fas, tumor necrosis factor receptor (TNF R55), and leukemia inhibiting factor receptor (LIF-R) after local irradiation. Adult male mice were locally irradiated on the testes. Induction of apoptosis in the different testicular cell types following X-ray radiation was identified by the TdT-mediated dUTP Nick End Labeling (TUNEL) approach. Growth factor expression was evidenced by semiquantitative RT-PCR and Western blot analyses. Apoptosis, identified through the TUNEL approach, occurred in radiosensitive testicular (premeotic) germ cells with the following kinetics: the number of apoptotic cells increased after 24 h (p < 0.001) and was maximal 48 h after a 2-Gy ionizing radiation (p < 0.001). Apoptotic cells were no longer observed 72 h after a 2-Gy irradiation. The number of apoptotic cells increased with the dose of irradiation (1-4 Gy). In the seminiferous tubules, the growth factor expression in premeiotic radiosensitive germ cells was m...Continue Reading
References
Jan 1, 1975·Current Topics in Molecular Endocrinology·V HanssonS N Nayfeh
May 7, 1976·Analytical Biochemistry·M M Bradford
May 1, 1975·Molecular and Cellular Endocrinology·L HagenäsS N Nayfeh
Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Apr 30, 1991·Biochemical and Biophysical Research Communications·P RossiR Geremia
Oct 1, 1989·International Journal of Andrology·A M ToeboschJ A Grootegoed
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D E HallahanR R Weichselbaum
Dec 1, 1985·International Journal of Andrology·J I DelicS M Shalet
Jul 1, 1970·The American Journal of Anatomy·M Dym, Y Clermont
Nov 1, 1983·Journal of Andrology·D K Clifton, W J Bremner
Jan 1, 1995·Biology of Reproduction·M DymN Ravindranath
Dec 1, 1994·The Journal of Cell Biology·S EstusE M Johnson
Feb 9, 1995·The New England Journal of Medicine·A S Lichter, T S Lawrence
Jun 1, 1993·Biology of Reproduction·L A CookerE Goldberg
Jun 1, 1996·Biology of Reproduction·M KangasniemiM L Meistrich
Jul 1, 1996·Biology of Reproduction·E K AllardK Boekelheide
Oct 1, 1996·The Journal of Cell Biology·M Oulad-AbdelghaniP Chambon
Feb 1, 1997·Biology of Reproduction·V CaussanelM Benahmed
Aug 15, 1997·Nucleic Acids Research·D MichelG Brun
Dec 17, 1998·Zygote : the Biology of Gametes and Early Embryos·G Durcova-HillsG Brem
Dec 22, 1998·Histochemistry and Cell Biology·R OlasoR Habert
Apr 15, 1999·International Journal of Radiation Biology·N GuittonC Chenal
Aug 24, 1999·Pharmacology & Therapeutics·J R Woodburn
Sep 1, 1999·Oncogene·D B ReardonR K Schmidt-Ullrich
Oct 3, 1999·The Journal of Biological Chemistry·P De CesarisE Ziparo
Feb 9, 2000·Investigational New Drugs·S M Huang, P M Harari
Apr 13, 2000·Radiation Research·M HaganP Dent
Aug 15, 2000·Molecular and Cellular Endocrinology·W YanJ Toppari
Citations
May 7, 2010·Journal of Molecular Histology·Mehmet KanterSule Parlar
Jul 24, 2013·Journal of Assisted Reproduction and Genetics·Mohammadreza GholamiJavad Mohammadi-Asl
Apr 26, 2002·Vaccine·Roberto Mitsuyoshi HiramotoHeitor Franco de Andrade
Jul 9, 2004·Biology of Reproduction·Maarit AhtiainenIlpo Huhtaniemi
Feb 14, 2009·Pharmacogenetics and Genomics·Markus A SchirmerJürgen Brockmöller
Jun 26, 2009·Reproductive Biology and Endocrinology : RB&E·Imen HammamiClaire Mauduit
Oct 25, 2013·International Journal of Biological Sciences·Liren QianJianming Cai
Sep 25, 2012·Basic & Clinical Pharmacology & Toxicology·Zhitao JiangDapeng Jiang
Jun 26, 2012·Environmental Toxicology·Dev Dutt PatelRaj Kumar
Apr 12, 2012·Radiation Research·Yukihiro FurusawaTakashi Kondo
Sep 25, 2004·FEBS Letters·Yun-Jung ChoiJin-Hoi Kim
Jun 20, 2007·International Journal of Radiation Biology·Sonia ChaterClaire Mauduit
Dec 14, 2004·Endocrinology·Anne FlorinMohamed Benahmed
Apr 12, 2003·Melanoma Research·Frédéric MouriauxSimon Saule
Nov 2, 2019·The Journal of Pharmacy and Pharmacology·Rasha Y M IbrahimWafaa M Elkady
Aug 27, 2014·Cancer Immunology Research·Megan E NellesChristopher J Paige
Oct 26, 2021·International Journal of Radiation Biology·Ahmed I SemaidaShady A Elfouly