Differential expression of heat shock 70 proteins in primary cultures from rat cerebellum

Brain Research
P J VoisinM Morrison-Bogorad

Abstract

While a number of studies have described the heat shock response in established cell lines and in primary cultures of cells derived from the nervous system, there has been no systematic analysis comparing expression and localization of the inducible heat shock 70 (hsp70) proteins and the constitutively synthesized members of the family (hsc70) in neurons and glia. In the present communication, we utilized specific probes to compare the expression of hsp70 and hsc70 mRNAs and proteins in two types of primary cultures, astroglial and neuro-astroglial, from postnatal rat cerebellum. Conditions were adjusted to maintain physiological numbers of microglia in both types of culture, and cultures were analyzed at a number of different time points following a precisely defined heat shock. The northern, in situ hybridization and immunohistochemical analyses resulted in a number of novel observations concerning the nature of the heat shock response in these neuronal and glial cells. In postnatal day 4-5 cultures, hsp70 mRNA levels were elevated for at least 10 h in both types of culture, but in situ hybridization analysis showed no evidence for hsp70 mRNAs in neurons. Microglia were the only cell type in which hsp70 was detected in non-st...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1992·Brain Research. Molecular Brain Research·R N NishimuraK B Clegg
Mar 1, 1992·Brain Research. Molecular Brain Research·M L WongM A Smith
Sep 1, 1992·Brain Research. Brain Research Reviews·B J Snider, M Morrison-Bogorad
Jan 1, 1992·Acta Neuropathologica·Y LiS R Levine
Oct 1, 1992·Experimental Cell Research·K Ohtsuka, A Laszlo
Dec 1, 1992·Journal of Neuroimmunology·M S FreedmanJ P Antel
Nov 1, 1992·Neurobiology of Aging·S PardueM Morrison-Bogorad
Jan 2, 1992·Nature·M J Gething, J Sambrook
Jan 1, 1991·Brain Research. Molecular Brain Research·R N NishimuraJ de Vellis
Mar 1, 1991·Journal of Neuroscience Research·B E DwyerK B Clegg
Apr 1, 1991·Neuropathology and Applied Neurobiology·R N NishimuraR Cole
Jan 1, 1990·Journal of Molecular Neuroscience : MN·S C Lin, M Morrison-Bogorad
Oct 15, 1990·Biochemical and Biophysical Research Communications·J J SaukM J Somerman
Feb 1, 1989·The Journal of Cell Biology·K L MilarskiR I Morimoto
Jan 1, 1988·Glia·W J StreitG W Kreutzberg
May 1, 1988·Journal of Neuroscience Research·R N NishimuraK Liotta
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J B LawrenceG S Stein
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K L Milarski, R I Morimoto
Apr 1, 1987·Journal of Neurocytology·W J Streit, G W Kreutzberg
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A Laszlo, G C Li
Jan 15, 1986·Brain Research·M TytellR J Lasek
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt
Jul 1, 1983·Journal of Neurochemistry·B R PearceF P White
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G C Li, Z Werb

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Citations

Jul 5, 2005·Journal of Neurochemistry·Tatiana V NovoselovaIrina V Guzhova
Aug 27, 2019·Frontiers in Immunology·Martin BuschArnd Heiligenhaus
Feb 25, 2000·Brain Research. Molecular Brain Research·D A Bechtold, I R Brown
Jan 18, 2006·Neurobiology of Aging·S PardueM Morrison-Bogorad
Jun 15, 2005·Journal of Proteome Research·Jae-Won YangGert Lubec

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