Abstract
Hepatic fibrosis leading to cirrhosis is the major morbidity in patients with biliary atresia (BA). This fibrosis is due to an imbalance in extracellular matrix (ECM) breakdown and deposition. We have previously demonstrated increased mRNA expression for inhibitors of ECM breakdown without increased expression for mediators of ECM deposition in our animal model of BA by d 14. However, only a mild degree of hepatic fibrosis was seen at this time. We hypothesized that expression patterns for these proteins may change once more significant fibrosis had been established, and added resuscitation to the model to improve survival. Interestingly, we found that some mice spontaneously recovered at later time points with resuscitation, and thus compared expression for inhibitors of ECM breakdown and deposition in sick and recovered mice to determine the differences. Newborn Balb/c mice received an intraperitoneal injection 1.0 x 10(6) fluorescence forming units of rhesus rotavirus 24h after birth. Mice were monitored daily for weight gain, development of jaundice, acholic stools, and bilirubinuria. Fifty muL/g of 5% dextrose in normal saline were subcutaneously injected daily to each mouse starting on d 7 until sacrifice. Mice that survi...Continue Reading
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Citations
Aug 31, 2013·Orphanet Journal of Rare Diseases·Claus Petersen, Mark Davenport
Mar 22, 2013·PloS One·Timo RathElke Roeb
Jul 18, 2012·Seminars in Pediatric Surgery·Claus Petersen
Mar 19, 2015·Journal of Pediatric Surgery·Claudia M G Keyzer-DekkerJ B F Hulscher
Jun 24, 2015·BioMed Research International·Yu-Wen Chung-DavidsonWeiming Li
Oct 8, 2013·Journal of Pediatric Surgery·Tatiana IordanskaiaEvan P Nadler
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Apr 4, 2018·Innovative Surgical Sciences·Claus Petersen, Omid Madadi-Sanjani
Mar 12, 2019·The Journal of Pediatrics·Jia-Feng WuMei-Hwei Chang