PMID: 9659292Jul 11, 1998Paper

Differential expression of IGF-I and IGF-binding protein-1 and -2 in periportal and perivenous zones of rat liver

The Journal of Endocrinology
S J HazelM Schalling

Abstract

IGF-I has important roles in regulating growth and metabolism. Circulating IGF-I is bound to specific binding proteins (IGFBP-1 to -6), with hepatocytes containing IGF-I, IGFBP-1 and -2 mRNA. Although many hepatic proteins are regionally expressed in the liver acinus, no studies have reported zonation of IGF protein expression. In this study we investigated the pattern of hepatic mRNA for the IGF proteins, vs the previously reported pepriportal gradient of phosphoenolpyruvate carboxykinase (PEPCK) expression. In situ hybridisation was used to analyse IGF-I, IGFBP-1, -2 and PEPCK mRNA in female Sprague-Dawley rats fed diets containing low (6%), normal (21%) or high (35%) protein. We report for the first time that IGFBP-1 and -2 and IGF-I are differentially expressed in the liver acinus. In the normal- and high-protein groups, levels of IGFBP-1 mRNA were higher in the perivenous region, i.e. the opposite gradient to PEPCK, with a higher gradient of IGFBP-1 expression in the high-protein group. In contrast, IGFBP-2 had a similar pattern to PEPCK, and a periportal gradient of IGF-I mRNA was also seen in the low-protein group. Using computerised image analysis, levels of IGFBP-1 and -2 mRNA were elevated 2- and 10-fold respectively,...Continue Reading

Citations

Aug 21, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Olgica NedićNatasa Ilić
Mar 6, 2004·Seminars in Neonatology : SN·S V Beath
Dec 7, 2007·The Journal of Pharmacology and Experimental Therapeutics·Iain A Murray, Gary H Perdew
Jan 28, 2017·Redox Biology·Thomas Kietzmann
Mar 11, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·E M ZimmermannP K Lund
May 15, 2019·International Journal of Molecular Sciences·Thomas Kietzmann
Nov 16, 2004·Journal of Clinical Laboratory Analysis·Olgica NedićNatasa Ilić

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