PMID: 11903621Mar 21, 2002Paper

Differential expression of individual gene copies from within a tRNA multigene family in the mulberry silkworm Bombyx mori

Insect Molecular Biology
L Srinivasan, Karumathil P Gopinathan

Abstract

In mulberry silkworm Bombyx mori, tRNA1(Gly) constitutes a multigene family from which the individual members are transcribed at different levels in vitro in homologous nuclear extracts. We report here the quantification of functional transcripts of these gene copies in vivo in B. mori-derived BmN cells based on a suppression assay. The gene copies were converted to encode suppressor tRNAs and co-transfected into cell lines with reporter gene(s) harbouring one or more nonsense mutations and the reporter gene activity was quantified. Individual members of the gene family were transcribed to very high-, medium- and very low-levels, following the same pattern as in vitro. All these gene copies were maximally expressed in Bm cells as compared to other insect cell lines.

References

Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R TanejaK P Gopinathan
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L S YoungK U Sprague
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Jun 28, 2000·Nucleic Acids Research·C Z ZhouZ G Li

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Citations

Apr 20, 2002·The Biochemical Journal·Lakshmi Srinivasan, Karumathil P Gopinathan
Jun 18, 2005·Genome Biology·Gavin C Conant, Andreas Wagner
Oct 20, 2006·International Journal of Radiation Biology·H B ManjunathaS R Inamdar
Mar 13, 2016·Trends in Parasitology·Petra MatouškováLenka Skálová
Jul 14, 2005·The Biochemical Journal·Akhila Parthasarthy, Karumathil P Gopinathan
Feb 11, 2021·Wiley Interdisciplinary Reviews. RNA·Joseph J PorterJohn D Lueck

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