Differential expression of individual suppressor tRNA(Trp) gene gene family members in vitro and in vivo in the nematode Caenorhabditis elegans.

Molecular and Cellular Biology
L LiB M Honda

Abstract

Eight different amber suppressor tRNA (suptRNA) mutations in the nematode Caenorhabditis elegans have been isolated; all are derived from members of the tRNA(Trp) gene family (K. Kondo, B. Makovec, R. H. Waterston, and J. Hodgkin, J. Mol. Biol. 215:7-19, 1990). Genetic assays of suppressor activity suggested that individual tRNA genes were differentially expressed, probably in a tissue- or developmental stage-specific manner. We have now examined the expression of representative members of this gene family both in vitro, using transcription in embryonic cell extracts, and in vivo, by assaying suppression of an amber-mutated lacZ reporter gene in animals carrying different suptRNA mutations. Individual wild-type tRNA(Trp) genes and their amber-suppressing counterparts appear to be transcribed and processed identically in vitro, suggesting that the behavior of suptRNAs should reflect wild-type tRNA expression. The levels of transcription of different suptRNA genes closely parallel the extent of genetic suppression in vivo. The results suggest that differential expression of tRNA genes is most likely at the transcriptional rather than the posttranscriptional level and that 5' flanking sequences play a role in vitro, and probably i...Continue Reading

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Citations

Jun 23, 2001·Journal of Molecular Biology·E P Geiduschek, G A Kassavetis
Feb 22, 2014·Annual Review of Biochemistry·Jason W Chin
May 2, 2006·Molecular and Cellular Biology·Robyn BranickySiegfried Hekimi
Apr 15, 2004·Molecular and Cellular Biology·Yung-Shu KuanLillie L Searles
Aug 26, 2016·PLoS Genetics·Dror SagiOded Rechavi
Feb 11, 2021·Wiley Interdisciplinary Reviews. RNA·Joseph J PorterJohn D Lueck
Mar 7, 2021·International Journal of Molecular Sciences·Frida BelinkyIgor B Rogozin
Aug 9, 2011·Journal of the American Chemical Society·Sebastian Greiss, Jason W Chin

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