Differential expression of intestinal nutrient transporters and host defense peptides in Eimeria maxima-infected Fayoumi and Ross chickens

Poultry Science
S SuE A Wong

Abstract

Fayoumi chickens are believed to be more disease resistant compared to commercial broiler chickens. The objective of this study was to compare mRNA expression of intestinal nutrient transporters, digestive enzymes, and host defense peptides (HDP) between Eimeria maxima-challenged Fayoumi and Ross broiler chickens. At 21 d of age, Ross broilers and Fayoumi lines M5.1 and M15.2 were challenged with 1,000 E. maxima oocysts. Control birds were not challenged. Duodenum, jejunum, and ileum were sampled (n = 6) at 7 d post challenge. Gene expression was analyzed using relative quantification PCR. Data were analyzed by ANOVA and significance level was set at P < 0.05. There was numerical, but not statistically significant, differential weight gain depression for Ross (15%) and Fayoumi lines M5.1 (21%) and M15.2 (22%) and significant line-specific changes in gene expression. For nutrient transporters, there was downregulation of mRNA for the brush border membrane, amino acid transporters b0,+AT/rBAT, BoAT, and EAAT3 in different segments of the small intestine of Ross and both lines of Fayoumi chickens, indicating that E. maxima challenge likely caused a decrease in nutrient uptake. For HDP, there was downregulation of avian beta defens...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 25, 2002·Avian Pathology : Journal of the W.V.P.A·Mohamed K HassanMona M Aly
Aug 2, 2003·The Journal of Parasitology·R H Fetterer, R C Barfield
Jul 21, 2009·Biochemical and Biophysical Research Communications·Claire L TownesJudith Hall
Dec 24, 2010·Experimental Parasitology·Lindsay H SumnersRami A Dalloul
Apr 5, 2011·Veterinary Medicine International·Nedyalka V GeorgievaZvezdelina Yaneva
Apr 25, 2012·Amino Acids·John T Brosnan, Margaret E Brosnan
Mar 20, 2013·Molecular Aspects of Medicine·Yoshikatsu KanaiMatthias A Hediger
Mar 20, 2013·Molecular Aspects of Medicine·Dimitrios FotiadisManuel Palacín
May 7, 2013·Developmental and Comparative Immunology·Tryntsje CuperusHenk P Haagsman
Mar 4, 2014·Pharmaceuticals·Guolong Zhang, Lakshmi T Sunkara
Dec 15, 2015·Frontiers in Veterinary Science·Kelsy RobinsonGuolong Zhang

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