Differential expression of isoforms of PSD-95 binding protein (GKAP/SAPAP1) during rat brain development

FEBS Letters
N KawashimaK Sobue

Abstract

PSD-95/SAP90, which binds to the C-terminus of NMDA receptor and Shaker-type potassium channel, is one of the major postsynaptic density proteins. Recently, novel classes of proteins interacting with the guanylate kinase domain of PSD-95 have been identified, guanylate kinase-associated protein (GKAP) and SAP90/PSD-95-associated proteins (SAPAPs). Here we report the isolation of new isoforms of PSD-95 binding protein (GKAP/SAPAP1) using the yeast two-hybrid system. The isolated protein directly interacts with the guanylate kinase domain of PSD-95. Northern blot analyses revealed that the expression of these isoforms containing distinct N-terminal sequences is differentially regulated during brain development. The present findings suggest that each isoform of the PSD-95 binding protein is differentially expressed in a development-dependent manner and may be involved in the complex formation of PSD-95 and channel/receptors at the postsynaptic density.

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Citations

Apr 29, 2005·Brain Research. Molecular Brain Research·Zhang Jing-PingTatsuo Suzuki
Nov 9, 2006·PLoS Biology·Noah W GrayKarel Svoboda
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·S TadokoroK Sobue
Apr 10, 2014·The European Journal of Neuroscience·Eoin C O'ConnorCamilla Bellone
Sep 4, 2012·Psychiatry Research·Jun-Ming LiChia-Hsiang Chen
May 8, 2015·AIDS Research and Human Retroviruses·Sumonmal UttayamakulTatsuo Shioda
Oct 2, 2009·The International Journal of Neuropsychopharmacology·Yael Chertkow-DeutsherDorit Ben-Shachar
Jul 21, 2004·Gene Expression Patterns : GEP·Daimark Bennett, Luke Alphey
Jan 14, 2003·The Journal of Biological Chemistry·Shinichi UsuiKenji Sobue
Dec 3, 1999·Journal of Neurochemistry·D G StathakisK Forsman-Semb
Jan 9, 2021·Virchows Archiv : an International Journal of Pathology·Tomohiko IshikawaToru Furukawa

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