PMID: 8946388Nov 25, 1996Paper

Differential expression of Leishmania major beta-tubulin genes during the acquisition of promastigote infectivity

Molecular and Biochemical Parasitology
Richard M R CoulsonJ W Ajioka

Abstract

Tubulin expression has been analysed as the insect stage of the protozoan parasite Leishmania major differentiates from a non-infective to an infective form. This transformation of the promastigote stage occurs in vitro and analysis of beta-tubulin mRNA expression in axenically grown promastigotes showed that a 2200 nt transcript is predominately expressed in non-infective promastigotes. The message contains a motif associated with mRNA intracellular localisation and its level is reduced by an order of magnitude in infective promastigotes through a mechanism involving RNA stability. A 3200 nt RNA, the major beta-tubulin transcript in the infective stage, is encoded by a single copy gene at the 3' end of the array that encodes the 2200 nt RNA. These RNAs, as well as a gene encoding a beta-tubulin transcript highly up-regulated in the mammalian stage of the parasite, encode polypeptides that are apparently functionally equivalent but have highly diverged 3' untranslated regions. This differential regulation of the dispersed isogenes may reflect the involvement of a mechanism altering tubulin synthesis during the Leishmania life cycle. The analysis of alpha-tubulin RNA levels revealed the abundance of this message falls as promast...Continue Reading

References

Apr 1, 1990·Molecular and Biochemical Parasitology·R M Coulson, D F Smith
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Mar 30, 1984·Science·D L Sacks, P V Perkins
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D Fong, K P Chang
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·A HehlI Roditi
Feb 1, 1990·Parasitology Today·T SeebackD Lawson

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Citations

Jul 15, 2003·Experimental Parasitology·Daniella C BartholomeuSantuza M R Teixeira
Aug 9, 2003·International Journal for Parasitology·Sabina I BelliNicolas Fasel
Feb 13, 2001·Molecular and Biochemical Parasitology·A BrittinghamM E Wilson
Apr 4, 2003·Molecular and Biochemical Parasitology·M P IribarA K Cruz
Feb 13, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Renata AlmeidaJenefer M Blackwell
Feb 17, 1999·Experimental Parasitology·S BelliN Fasel
Jul 8, 2015·Nucleic Acids Research·Laura A L DillonNajib M El-Sayed
Apr 25, 2002·Experimental Parasitology·J A BellatinW R McMaster
Nov 5, 1999·Annual Review of Microbiology·K Gull
Oct 20, 2005·International Journal for Parasitology·Rosiane A da SilvaSantuza M R Teixeira
Feb 10, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S M Teixeira
Aug 22, 2006·Journal of Enzyme Inhibition and Medicinal Chemistry·Abdala HiamPatrice Le Pape
Mar 26, 2002·The Journal of Biological Chemistry·Nathalie BoucherBarbara Papadopoulou
Jul 19, 2018·Frontiers in Microbiology·Roma SinhaNahid Ali
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·P ProbstJ R Webb
Sep 1, 2019·Trends in Parasitology·Amy N Sinclair, Christopher L de Graffenried
Mar 7, 2021·Genes·Luis E Florencio-MartínezSantiago Martínez-Calvillo

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