Differential expression of metastasis-associated cell surface glycoproteins and mRNA in a murine large cell lymphoma

Journal of Cellular Biochemistry
G L NicolsonV Rotter

Abstract

A metastatic variant cell subline of the Abelson virus-transformed murine large lymphoma/lymphosarcoma RAW117 has been selected in vivo ten times for liver colonization. Highly metastatic subline RAW117-H10 forms greater than 200 times as many gross surface liver tumor nodules as the parental line RAW117-P. Analysis of cellular proteins and glycoproteins indicates reduced expression of murine Moloney leukemia virus-associated p15, p30, and gp70, and increased expression of a sialoglycoprotein, gp150, in the highly metastatic H10 cells. Northern analyses of oncogene expression suggested that mRNA of various oncogenes was expressed equally or not expressed in the RAW117 cells of differing metastatic potential. Differential gene expression was examined using a cDNA library of 17,600 clones established from poly A+ mRNA isolated from H10 cells. The cDNA library was screened by the colony hybridization technique using probes made from both RAW117-P and -H10 cells. Approximately 99.5% of these cDNA clones were expressed identically in P and H10 cells. Of the few differentially expressed cDNA clones (approx. 150/17,600), one-half of these were identified as Moloney leukemia virus sequences in a separate probing with a radiolabeled Mol...Continue Reading

References

May 1, 1975·Journal of the National Cancer Institute·W C RaschkeH Coon
Feb 15, 1985·International Journal of Cancer. Journal International Du Cancer·R M KrisA Raz
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Jun 4, 1974·Biochemistry·V GlisinC Byus
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jan 1, 1984·Cancer Metastasis Reviews·G L Nicolson
Apr 1, 1984·Clinical & Experimental Metastasis·G L Nicolson
Apr 1, 1983·Clinical & Experimental Metastasis·C L ReadingG L Nicolson
Jan 1, 1982·Cancer Metastasis Reviews·I R Hart
Jun 1, 1980·European Journal of Biochemistry·C Auffray, F Rougeon
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C L ReadingG L Nicolson
Jun 1, 1980·Gene·D Hanahan, M Meselson

❮ Previous
Next ❯

Citations

Jan 1, 1987·Clinical & Experimental Metastasis·S S JoshiK W Brunson
May 1, 1989·Clinical & Experimental Metastasis·M E BrackeM M Mareel
Jan 1, 1991·Clinical & Experimental Metastasis·S S JoshiK W Brunson
Jan 1, 1990·Molecular Aspects of Medicine·T N Dear, R F Kefford
Jul 8, 2008·International Immunopharmacology·Shantaram S JoshiXioujun Wang
May 1, 1987·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G L Nicolson, N L Rosenberg
Apr 1, 1988·Journal of Cellular Biochemistry·R A LaBicheG L Nicolson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.