Differential Expression of microRNAs in Thymic Epithelial Cells from Trypanosoma cruzi Acutely Infected Mice: Putative Role in Thymic Atrophy

Frontiers in Immunology
Leandra Linhares-LacerdaWilson Savino

Abstract

A common feature seen in acute infections is a severe atrophy of the thymus. This occurs in the murine model of acute Chagas disease. Moreover, in thymuses from Trypanosoma cruzi acutely infected mice, thymocytes exhibit an increase in the density of fibronectin and laminin integrin-type receptors, with an increase in migratory response ex vivo. Thymic epithelial cells (TEC) play a major role in the intrathymic T cell differentiation. To date, the consequences of molecular changes promoted by parasite infection upon thymus have not been elucidated. Considering the importance of microRNA for gene expression regulation, 85 microRNAs (mRNAs) were analyzed in TEC from T. cruzi acutely infected mice. The infection significantly modulated 29 miRNAs and modulation of 9 was also dependent whether TEC sorted out from the thymus exhibited cortical or medullary phenotype. In silico analysis revealed that these miRNAs may control target mRNAs known to be responsible for chemotaxis, cell adhesion, and cell death. Considering that we sorted TEC in the initial phase of thymocyte loss, it is conceivable that changes in TEC miRNA expression profile are functionally related to thymic atrophy, providing new clues to better understanding the mecha...Continue Reading

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Citations

Feb 13, 2016·Frontiers in Cellular and Infection Microbiology·Carla C JudiceFabio T M Costa
Dec 24, 2015·Frontiers in Immunology·Geraldo Aleixo PassosErnna Hérida Oliveira
Apr 12, 2016·Frontiers in Microbiology·Leandra Linhares-Lacerda, Alexandre Morrot
Mar 24, 2017·Archivum Immunologiae Et Therapiae Experimentalis·Abdur Rahman Ansari, Huazhen Liu
Nov 11, 2017·Annals of the New York Academy of Sciences·Mélanie A CronRozen Le Panse
Jun 27, 2020·Frontiers in Immunology·Mélanie A CronRozen Le Panse
Apr 6, 2017·Frontiers in Microbiology·Ethel Bayer-SantosJosé F da Silveira
Jan 8, 2020·Cells·Stephanie Maia AcuñaSandra Marcia Muxel
Oct 25, 2020·Nature Reviews. Immunology·Enrico VelardiMarcel R M van den Brink
Jun 12, 2021·Frontiers in Immunology·Mingli LuoXi Sun

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Methods Mentioned

BETA
FCS
FACS
PCR
flow cytometry

Software Mentioned

Microcosm Target
FACS Diva
Target Scan
miRNAMap
miRTarBase
Rgraphviz
R
miRanda
geNorm

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