Differential Expression of miRNAs in the Hippocampi of Offspring Rats Exposed to Fluorine Combined with Aluminum during the Embryonic Stage and into Adulthood

Biological Trace Element Research
Qi-Di GeTing-Xu Jin

Abstract

A previous study from our team found that continuous exposure to fluorine combined with aluminum (FA) impaired the neurobehavioral reflexes, spatial learning, and memory of offspring rats. To date, the specific mechanisms for these changes are unclear. Here, high-throughput sequencing was utilized to analyze the microRNA (miRNA) profile of the hippocampi in the offspring of rats exposed to FA during the embryonic stage and into adulthood through tap water supplemented with NaF and AlCl3 at concentrations of (0, 0); (60, 600); (120, 600); and (240, 600) mg/L, respectively. qRT-PCR was performed to validate the reliability of the sequence data. Twenty differentially expressed miRNAs were selected for further analysis using bioinformatics tools. Several genes related to neuromodulation were found to be regulated by miR-10a-5p, miR-34b-5p, and miR-182, which might be harmful to normal nerve function. The protein levels of brain-derived neurotrophic factor (BDNF) and tyrosine receptor kinase B (TrkB) in hippocampus were markedly downregulated. These data suggest that miR-10a-5p, miR-34b-5p, and miR-182 and BDNF-TrkB signaling pathway are involved in mechanisms of hippocampal damage in the offspring of rats exposed to FA. HIGHLIGHTS:...Continue Reading

References

Jul 4, 2007·Genes & Development·John F EtchbergerOliver Hobert
Sep 29, 2011·The EMBO Journal·Athanasios ZovoilisAndre Fischer
Aug 14, 2012·Neuron·Elizabeth McNeill, David Van Vactor
Aug 29, 2012·Annals of Neurology·Soon-Tae LeeJae-Kyu Roh
Apr 18, 2013·PloS One·Rachel Clemens GrishamTeresa Nicolson
Aug 13, 2013·Experimental Neurology·Erika Reime KinjoAlexandre Hiroaki Kihara
Dec 3, 2014·Neurotoxicology and Teratology·Anna L ChoiPhilippe Grandjean
Jul 21, 2015·The Science of the Total Environment·Clare DeathJasmin Hufschmid
Oct 27, 2015·Journal of Epidemiology·Zhipeng FanDianjun Sun
Jan 15, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kari PollockJan A Nolta
Feb 3, 2016·Journal of Alzheimer's Disease : JAD·Mingming ZhuCaiyun Zhong
Nov 5, 2016·Perspectives in Public Health·Chitta R ChowdhuryMartin Grootveld
Feb 18, 2016·The Journal of Cell Biology·Carlos A MatosAna Luísa Carvalho
Feb 24, 2016·Environmental Science and Pollution Research International·Shanti Lal Choubisa, Darshana Choubisa
Oct 30, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Azhwar RaghunathEkambaram Perumal
Aug 27, 2016·Indian Journal of Public Health·M N ShruthiB N Kishore Kumar
Oct 22, 2016·Journal of Alzheimer's Disease : JAD·Qian Cai, Prasad Tammineni
Mar 18, 2017·International Journal of Molecular Sciences·Nesli AvganLyn R Griffiths
Mar 24, 2017·Cell Death & Disease·Subhas Chandra BiswasLloyd A Greene

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Citations

Feb 23, 2019·Environmental Science and Pollution Research International·Amal A Kinawy
Apr 14, 2020·International Journal of Environmental Health Research·Anqi WangYue Ba
Aug 24, 2021·Ecotoxicology and Environmental Safety·Zhongbi PengChun Xie

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Methods Mentioned

BETA
electrophoresis
PCR
CLIP-seq
transfection
RNA-seq

Software Mentioned

GraphPad Prism
SPSS
ImageJ
miRDB
miRanda
TargetScan
Bioconductor edgeR

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