Differential expression of multiple genes during articular chondrocyte redifferentiation

The Anatomical Record
D R HaudenschildF Binette

Abstract

Articular chondrocytes undergo a rapid change in phenotype and gene expression, termed dedifferentiation, when isolated from cartilage tissue and cultured on tissue culture plastic. On the other hand, "redifferentiation" of articular chondrocytes in suspension culture is characterized by decreased cellular proliferation and the reinitiation of synthesis of hyaline articular cartilage extracellular matrix molecules. The molecular triggers for these events have yet to be defined. Subtracted cDNA libraries representing genes involved in the early events of adult human articular chondrocyte redifferentiation were generated from human articular chondrocytes that were first cultured in monolayer, and subsequently transferred to suspension culture at 10(6) cells/ml for redifferentiation. Differential regulation of genes involved in cellular organization, nuclear structure, cellular growth regulation, and extracellular matrix deposition and remodeling were observed within 48 hr of this transfer. Many of these genes had not been previously identified in the chondrocyte differentiation pathway and a number of the isolated cDNAs did not have homologies to sequences in the public data banks. Genes involved in IL-6 signal transduction inclu...Continue Reading

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Citations

Jun 26, 2007·Tissue Engineering·Stephen RapkoSven Olek
Jul 7, 2012·Tissue Engineering. Part a·Derek F AmanatullahDominik R Haudenschild
Feb 21, 2006·Journal of Biomedicine & Biotechnology·Vega Villar-SuárezJ M Villar-Lacilla
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