Differential expression of myogenic regulatory factor MyoD in pacu skeletal muscle (Piaractus mesopotamicus Holmberg 1887: Serrasalminae, Characidae, Teleostei) during juvenile and adult growth phases

Micron : the International Research and Review Journal for Microscopy
Fernanda Losi Alves de AlmeidaMaeli Dal Pai-Silva

Abstract

Skeletal muscle is the edible part of the fish. It grows by hypertrophy and hyperplasia, events regulated by differential expression of myogenic regulatory factors (MRFs). The study of muscle growth mechanisms in fish is very important in fish farming development. Pacu (Piaractus mesopotamicus) is one of the most important food species farmed in Brazil and has been extensively used in Brazilian aquaculture programs. The aim of this study was to analyze hyperplasia and hypertrophy and the MRF MyoD expression pattern in skeletal muscle of pacu (P. mesopotamicus) during juvenile and adult growth stages. Juvenile (n=5) and adult (n=5) fish were anaesthetized, sacrificed, and weight (g) and total length (cm) determined. White dorsal region muscle samples were collected and immersed in liquid nitrogen. Transverse sections (10 microm thick) were stained with Haematoxilin-Eosin (HE) for morphological and morphometric analysis. Smallest fiber diameter from 100 muscle fibers per animal was calculated in each growth phase. These fibers were grouped into three classes (<20, 20-50, and >50 microm) to evaluate hypertrophy and hyperplasia in white skeletal muscle. MyoD gene expression was determined by semi-quantitative RT-PCR. PCR products w...Continue Reading

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Citations

Sep 4, 2009·Biological Reviews of the Cambridge Philosophical Society·Nasser Al-Shanti, Claire E Stewart
Aug 3, 2010·Micron : the International Research and Review Journal for Microscopy·Fernanda Losi Alves de AlmeidaMaeli Dal Pai-Silva
Feb 28, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Aline Cristina MichelinMaeli Dal-Pai-Silva
Dec 25, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tassiana Gutierrez de PaulaMaeli Dal-Pai-Silva
Apr 4, 2021·International Journal of Molecular Sciences·Bruna Tereza Thomazini ZanellaMaeli Dal-Pai-Silva
May 12, 2019·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Rondinelle Artur Simões SalomãoMaeli Dal-Pai-Silva
Nov 6, 2021·Biological Trace Element Research·Md Fazle RohaniMd Shahjahan

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