Differential expression of proteins in fetal brains of alcohol-treated prenatally C57BL/6 mice: a proteomic investigation.

Electrophoresis
Y SariYehia Mechref

Abstract

Alcohol is known to impede the growth of the central nervous system and to induce neurodegeneration through cellular apoptosis. We have previously shown that moderate prenatal alcohol exposure results in brain defects at different stages of development. In this study, we further characterize the proteomic architecture underlying ethanol teratogenesis during early fetal brain development using chromatography in conjunction with a LC-MS/MS system. Pregnant C57BL/6 mice were exposed from embryonic day 7 (E7) to E13 with either a 25% ethanol derived calorie or pair-fed liquid diets. At E13, fetal brains were collected from five dams for each group. Individual brains were homogenized and the extracted proteins were then tryptically digested and analyzed by LC-MS/MS. Label-free quantitative proteomic analyses were performed on proteomes extracted from fetal brains of both alcohol-treated (ALC) and pair-fed groups. These analyses demonstrated that prenatal alcohol exposure induced significant downregulation (p<0.001) of the expression of mitochondrial enzymes including ADP/ATP translocase 1, ATP synthase subunit alpha and ubiquinol-cytochrome-c reductases. In addition, mitochondrial carrier homolog 1, which plays a role in apoptosis, ...Continue Reading

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Citations

Aug 1, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Giorgio GoriniR Dayne Mayfield
Oct 25, 2011·Journal of Biomedical Science·Youssef SariDragan Isailovic
Jan 13, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ping ZhouCostantino Iadecola
Aug 16, 2011·Journal of Proteomics·Jayanth Ramadoss, Ronald R Magness
Nov 17, 2015·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Keiko MashimoYoukichi Ohno
Jan 17, 2012·Proteomics·Michaela D FiliouChristoph W Turck
Jul 26, 2018·Neurochemical Research·Mohammed A KashemVladimir J Balcar
Jun 20, 2018·Journal of Molecular Neuroscience : MN·Patricia C SwartJacqueline J Dimatelis
Jan 17, 2021·International Journal of Molecular Sciences·Laura Almeida-ToledanoMaría Dolores Gómez-Roig

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