Differential expression of rat insulin I and II messenger ribonucleic acid after prolonged exposure of islet beta-cells to elevated glucose levels

Endocrinology
Z LingD G Pipeleers

Abstract

Prolonged exposure of rat islet beta-cells to 10 mmol/liter glucose has been previously shown to activate more cells into a glucose-responsive state (>90%) than has exposure to 6 mmol/liter glucose (50%). The present study demonstrates that this recruitment of more activated cells results in 4- to 6-fold higher levels of proinsulin I and proinsulin II messenger RNA (mRNA). However, only the rate of proinsulin I synthesis is increased. Failure to increase the rate of proinsulin II synthesis in the glucose-activated cells results in cellular depletion of the insulin II isoform, which can be responsible for degranulation of beta-cells cultured at 10 mmol/liter glucose. Higher glucose levels (20 mmol/liter) during culture did not correct this dissociation between the stimulated insulin I formation and the nonstimulated insulin II formation. On the contrary, the rise from 10 to 20 mmol/liter glucose resulted in a 2-fold reduction in the levels of proinsulin II mRNA, but not of proinsulin I mRNA; this process further increased the ratio of insulin I over insulin II to 5-fold higher values than those in freshly isolated beta-cells. The present data suggest that an elevated insulin I over insulin II ratio in pancreatic tissue is a mark...Continue Reading

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Citations

Jul 3, 2010·Biochemical and Biophysical Research Communications·Ji Hoon YangHoon Taek Lee
Apr 27, 2010·Analytical Biochemistry·Farah T van GenderenChris Van Schravendijk
Nov 5, 2002·Biochemical and Biophysical Research Communications·Richard BarryIrene C Green
Aug 15, 2002·Biochemical and Biophysical Research Communications·Helen Roderigo-MilnePeter M Jones
Nov 22, 2013·Cell Transplantation·Ana Lucia Campanha-RodriguesMari C Sogayar
Apr 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Man-Xia LuEun-Hee Hwang
Nov 27, 2020·Cellular and Molecular Life Sciences : CMLS·Essam M Abdelalim
Dec 19, 2003·Biochemical and Biophysical Research Communications·Miwako KajiharaSatoru Takahashi
Apr 9, 2010·Cell Metabolism·Chunyan GuJacqueline E Lee

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