PMID: 30141876Dec 1, 2016Paper

Differential Expression of Six wnt Gene Family Members in Echinococcus granulosus Protoscoleces and Adult Worms

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Zheng-rong WangChun-sheng Xu

Abstract

To investigate the differential mRNA expression and tissue distribution of wnt [wingless-type mouse mammary tumor virus (MMTV) integration site family, wnt] gene members wnt1, wnt2, wnt4, wnt5, wnt11A and wnt11B in protoscoleces and adult worms of Echinococcus granulosus. The mRNA expression of wnt1, wnt2, wnt4, wnt5, wnt11A and wnt11B was determined by qRT-PCR. Tissue distribution of wnt1, wnt2, wnt4, wnt5, wnt11A and wnt11B in Echinococcus granulosus protoscoleces was determined by the whole-mount in situ hybridization. The qRT-PCR results showed that the mRNA expression levels of wnt1 and wnt2 in the adult worms were 1.49 (P>0.05) and 2.53 folds(P<0.05) of those in the protoscoleces, respectively. The mRNA expression levels of wnt4, wnt5, wnt11A and wnt11B in the protoscoleces were 25.00(P<0.01), 33.33(P<0.01), 14.29(P<0.01) and 1.03 folds(P>0.05) of those in the adult worms, respectively. In brief, there was no significant difference of mRNA expression in wnt2 and wnt11B between protoscoleces and adult, but there was a significant difference of mRNA expression in wnt1, wnt4, wnt5 and wnt11A between protoscoleces and adults. Results of the whole-mount in situ hybridization showed that in protoscoleces wnt1 was mainly loc...Continue Reading

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