Differential expression of specific FGF ligands and receptor isoforms during osteogenic differentiation of mouse Adipose-derived Stem Cells (mASCs) recapitulates the in vivo osteogenic pattern

Gene
Natalina Quarto, Michael T Longaker

Abstract

The ability of Adipose-derived Stem Cells (ASCs) to differentiate into various tissues in vitro and in vivo, a function known as "stem cell plasticity", makes them an appealing cell source for tissue engineering. Our laboratory is particularly focused on the potential role of adipose tissue as a readily available postnatal source of osteoprogenitor. Fibroblast growth factors (FGF) and their receptors (FGFR) are important regulators of osteogenesis. The goal of this study was to elucidate how changes in temporal expression patterns of individual components of the fibroblast growth factor (FGF) signaling axis correlate with osteogenic differentiation of mASCs. Our results indicate that FGF ligand genes, such as Fgf-2, -4, -8, and -18, displayed a differential and dynamic profile during mouse ASC (mASC) osteogenesis. Fgf-2 transcript was down-regulated, while Fgf-18 transcript level was strongly up-regulated. Interestingly, a drift in the ratio of different FGF-2 protein forms, with translation favoring the HMWFGF-2 forms, occurred during osteogenic differentiation, whereas, the expression of LMWFGF-2 form was down-regulated. This finding shares similarity with a previous study suggesting that preferential expression of the HMWFGF...Continue Reading

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Citations

Mar 2, 2011·In Vitro Cellular & Developmental Biology. Animal·T KizhnerE Livne
Jan 26, 2011·The Journal of Biological Chemistry·Matthew D KwanMichael T Longaker
Apr 20, 2014·International Journal of Molecular Sciences·Maria Giovanna ScioliAugusto Orlandi
Mar 6, 2009·Cell Adhesion & Migration·Atsushi Natsume, Jun Yoshida
Jan 5, 2011·Organogenesis·Kristine C RustadGeoffrey C Gurtner

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