Differential expression of the cellular retinaldehyde-binding protein in bovine ciliary epithelium

Experimental Eye Research
J M Martín-AlonsoM Coca-Prados

Abstract

Differential expression of the cellular retinaldehyde-binding protein (CRALBP), a likely component of the visual cycle, has been observed in the ocular ciliary epithelium, a bilayer of neurepithelial cells involved in the active transport of aqueous humor. Antibody probes raised to bovine ciliary epithelium were used to isolate a cDNA encoding CRALBP from a bovine ciliary epithelium cDNA expression library. Northern analysis of poly A+RNA isolated from three different regions of the neonate ciliary epithelium revealed a three-fold higher level of CRALBP expression in the most anterior portion of the pars plicata region relative to the posterior pars plana region. Western blot analysis using CRALBP-specific antibodies also demonstrated that the level of CRALBP expression was higher in the most anterior portion of the ciliary epithelium and gradually decreases toward the posterior region in both neonate and adult bovine eyes. Using indirect immunofluorescence and frozen-sectioned bovine ciliary epithelium, CRALBP was localized to the pigmented ciliary epithelial cell layer along the entire ciliary epithelium. In the nonpigmented ciliary epithelial cell layer, CRALBP was only detected in the pars plana region. Pigmented ciliary ep...Continue Reading

Citations

Jun 7, 2000·The Biochemical Journal·N Noy
Mar 31, 2011·The British Journal of Ophthalmology·Shagufta NazSheikh Riazuddin
Oct 3, 2012·PloS One·Sarah F JanssenArthur A B Bergen
Jun 8, 2001·Biochemical and Biophysical Research Communications·R Bertazolli-FilhoM Coca-Prados
Apr 7, 1999·Progress in Retinal and Eye Research·M Coca-PradosJ Ortego

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