Differential expression of the demosponge (Suberites domuncula) carotenoid oxygenases in response to light: protection mechanism against the self-produced toxic protein (Suberitine).

Marine Drugs
Werner E G MüllerHeinz C Schröder

Abstract

The demosponge Suberites domuncula has been described to contain high levels of a proteinaceous toxin, Suberitine, that displays haemolytic activityIn the present study this 7-8 kDa polypeptide has been isolated and was shown to exhibit also cytotoxic effects on cells of the same species. Addition of retinal, a recently identified metabolite of β-carotene that is abundantly present in S. domuncula was found to reduce both the haemolytic and the cell toxic activity of Suberitine at a molar ratio of 1:1. Spectroscopic analyses revealed that the interaction between β-carotene and Suberitine can be ascribed to a reversible energy transfer reaction. The enzyme that synthesises retinal in the sponge system is the β,β-carotene-15,15'-dioxygenase [carotene dioxygenase]. In order to clarify if this enzyme is the only β-carotene-metabolizing enzyme a further oxygenase had been identified and cloned, the (related) carotenoid oxygenase. In contrast to the dioxygenase, the carotenoid oxygenase could not degrade β-carotene or lycopene in Escherichia coli strains that produced these two carotenoids; therefore it had been termed related-carotenoid oxygenase. Exposure of primmorphs to light of different wavelengths from the visible spectrum res...Continue Reading

References

Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D J Jang, M A el-Sayed
Nov 16, 1987·European Journal of Biochemistry·Z KljajićW E Müller
Dec 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W E MüllerA Dorn
Dec 1, 1985·Analytical Biochemistry·S J Compton, C G Jones
Aug 1, 1972·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·W E MüllerR K Zahn
Jan 1, 1982·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·L CarielloL Zanetti
Mar 22, 2003·Die Naturwissenschaften·Werner E G MüllerHeinz C Schröder
Apr 25, 2003·Trends in Plant Science·Giovanni GiulianoJohannes von Lintig
Aug 12, 2003·The Journal of Experimental Biology·Matthias WiensWerner E G Müller
Mar 29, 2006·Advances in Biochemical Engineering/biotechnology·Ghosh DebashishMukherjee Joydeep
May 20, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Carsten EckertWerner E G Müller
Jan 20, 2009·Cellular and Molecular Life Sciences : CMLS·W E G MüllerM Wiens
Jul 18, 2009·Investigative Ophthalmology & Visual Science·Federico Gonzalez-FernandezDebashis Ghosh
Sep 11, 2010·Journal of Cellular Biochemistry·Matthias WiensWerner E G Müller

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Citations

Mar 12, 2013·Integrative and Comparative Biology·Werner E G MüllerXiaohong Wang
Aug 19, 2014·General and Comparative Endocrinology·A AndréM M Santos
Jun 27, 2020·Biomimetics·Hipassia M Moura, Miriam M Unterlass
Apr 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sheetal UppalEugenia Poliakov
Feb 20, 2015·The Journal of Experimental Biology·Sally P Leys

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Datasets Mentioned

BETA
FR848951
HE603239

Methods Mentioned

BETA
gel filtration
electrophoresis
ELISA
PCR

Software Mentioned

BLAST
Genedoc
iCycler
FASTA

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