Differential expression of the fibroin gene in developmental stages of silkworm, Antheraea mylitta (Saturniidae)

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
A DattaS C Kundu

Abstract

Fibroin gene expression during the larval developmental stages of the Saturniid silkworm, Antheraea mylitta, was analyzed. Northern blot analysis of larval silk gland total RNA using the fibroin gene as a probe showed that fibroin is expressed in the intermoult stages and repressed during the moulting stages. Abundance of fibroin transcripts gradually increased from the third to fifth intermoult stage, reaching a peak in the fifth intermoult. Transcripts declined during the early spinning stage. Western blot analysis of fibroin protein production with anti-fibroin antibody confirmed the differential fibroin expression, in accordance with fibroin mRNA synthesis. Dot blot hybridization of genomic DNA isolated from each larval developmental stage with the labelled fibroin gene showed that at the genomic level, the relative concentration of the fibroin gene was constant throughout the developmental stages. Our data confirm that fibroin gene expression in A. mylitta, like in B. mori, is transcriptionally controlled and shows differential temporal variations.

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Citations

Dec 9, 2003·Journal of Molecular Biology·Elisabetta BiniDavid L Kaplan
Dec 17, 2003·Prostaglandins & Other Lipid Mediators·Yun-Gen Miao, K Sashindran Nair
Feb 6, 2016·ACS Applied Materials & Interfaces·Rosalyn D AbbottDavid L Kaplan
Mar 14, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Rupesh DashS C Kundu
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Aug 14, 2021·Acta Biomaterialia·Prerak Gupta, Biman B Mandal
Aug 25, 2021·Biomedical Materials·Deboki NaskarSubhas C Kundu

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