Differential expression of the Gstp2 gene between the aboriginal species Mus spretus and the laboratory mouse Mus musculus

Mutation Research
Nieves AbrilCarmen Pueyo

Abstract

Glutathione S-transferases (GSTs) are pivotal phase-II enzymes for detoxification of xenobiotics. Pi-class GSTs play key roles in determining cancer susceptibility. The laboratory mouse Mus musculus (Mm) has two GST-Pi-encoding genes; while MmGstp1 is the counterpart of the unique human and rat Pi-class GST gene, the function of MmGstp2 remains unclear because its expression is almost undetectable in liver and its product lacks activity against typical GST/GST-Pi substrates. Mus spretus (Ms) is an aboriginal mouse species of great interest as a bio-indicator in environmental pollution studies and a reservoir of novel allelic variants and phenotypes. Using absolute real-time RT-PCR, we demonstrate significant differences in the hepatic levels of GST-Pi-encoding mRNAs between both mouse species. Particularly, we found that the Gstp2 gene of M. spretus, unlike its M. musculus counterpart, attains relatively high steady-state level of expression (∼30molecules/pg of total liver RNA in mice dwelling in a non-polluted area). To test whether the interspecies difference in Gstp2 mRNA levels is due, at least in part, to evolutionary divergence in the promoter regions, we (i) sequenced the 5'-flanking regulatory regions of the two Gstp2 g...Continue Reading

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Citations

Mar 19, 2014·Journal of Proteomics·Miguel Ángel García-SevillanoJosé Luis Gómez-Ariza
May 7, 2020·Food & Function·Noelia Morales-PrietoNieves Abril
Sep 30, 2020·Biology of Reproduction·Ayumi HasegawaAtsuo Ogura

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