PMID: 8972856Dec 1, 1996Paper

Differential expression of the human ST5 gene in HeLa-fibroblast hybrid cell lines mediated by YY1: evidence that YY1 plays a part in tumor suppression

Nucleic Acids Research
J H LichyM M Tsai

Abstract

Through a mutational analysis of a differentially regulated enhancer, we present evidence that supports a role for the transcription factor YY1 in tumor suppression in HeLa/fibroblast somatic cell hybrids. The human ST5 gene was previously shown to be expressed as three RNA species, 4.6, 3.1 and 2.8 kb in length. Whereas the two larger species are expressed at similar levels in all cell lines examined, the 2.8 kb mRNA is expressed specifically in non-tumorigenic hybrids. In this study, the basis for the differential expression of this mRNA species was investigated. The message was shown to originate from a promoter located within an intron of the ST5 gene. An enhancer located approximaely 1500 nt upstream of the start site was required for cell type specific expression. Mutational analysis of this enhancer revealed an AP1 site and five YY1 sites which were necessary for full enhancer activity. Levels of YY1 DNA binding activity were found to be as much as 6-fold higher in the non-tumorigenic cells relative to the tumorigenic cells, while AP1 activity was similar in both cell types. These results suggest that a signaling pathway targeting YY1 may play an important role in tumor suppression in HeLa-fibroblast hybrids.

References

Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M von Knebel DoeberitzH zur Hausen
Nov 11, 1995·Nucleic Acids Research·R P Hyde-DeRuyscherT Shenk
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Citations

Jan 18, 2012·Critical Reviews in Oncogenesis·Michael AtchisonMadhusudhan Papasani
Mar 22, 2006·Expert Opinion on Therapeutic Targets·Chi-Chung WangPan-Chyr Yang
Feb 26, 2015·The Journal of Cell Biology·Maria S IoannouPeter S McPherson
Aug 28, 2003·Cancer·Keith M Skubitz, Amy P N Skubitz
Apr 1, 1997·Neuron·T C Südhof
Jun 20, 1998·The Journal of Biological Chemistry·M MajidiJ H Lichy

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