PMID: 19912796Apr 1, 1991Paper

Differential expression of the rat homeobox genes in neural cell lines

Molecular and Cellular Neurosciences
S Y ChungD Hilt

Abstract

The mammalian homeobox genes are a family of genes that are expressed at high levels in the nervous system and encode sequence-specific DNA-binding proteins and transcriptional factors. To understand the expression and function of the homeobox genes in the nervous system, we have studied the expression of the rat homeobox genes in the RT4 cell lines, which mimic specific properties of the neuronal, glial, and their bipotential progenitor cell types. Northern blot analyses indicate that the rat homeobox R5 and Rlb genes are expressed in all cell types in the RT4 cell family. The sizes and levels of RNA transcripts are comparable to those observed in vivo in rat spinal cord and rat embryos. In contrast, another rat homeobox gene Rla, which is expressed in vivo and is closely linked to the R1b gene, does not express detectable RNA species in the RT4 cell family. Thus, the rat homeobox genes are differentially regulated in the RT4 cell family. In protein blotting analyses, anti-R5 peptide antisera react with a 60-kDa protein in cell extracts from all the RT4 cell lines. Immunofluorescence experiments demonstrate that the homeobox R5 gene product is present in all cell lines and that the protein is predominantly localized in the nuc...Continue Reading

References

Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·Y Tomozawa, N Sueoka
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 27, 1990·Science·M Kessel, P Gruss
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J DeschampsO Destrée
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M R Freeman, N Sueoka
May 1, 1988·Trends in Neurosciences·C D Stern, R J Keynes
Feb 1, 1989·Genes & Development·W F OdenwaldR A Lazzarini
Jul 28, 1989·Biochimica Et Biophysica Acta·M P ScottG W Hartzell
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A FainsodF H Ruddle
Jun 5, 1987·Science·W J Gehring
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M KesselP Gruss
Jan 1, 1988·Differentiation; Research in Biological Diversity·F MavilioP W Andrews
Jul 1, 1988·Genes & Development·P W Holland, B L Hogan
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.