Differential expression of urinary inter-alpha-trypsin inhibitor trimers and dimers in normal compared to active calcium oxalate stone forming men

The Journal of Urology
S R MarengoJ Y Chung

Abstract

We determine if the immunoreactive profile of urinary inter-alpha-trypsin inhibitor can be used to distinguish between normal individuals and individuals with calcium oxalate stone disease. Urinary proteins were dialyzed against water (15 kDa. molecular weight cutoff), lyophilized and resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (6% acrylamide, reducing conditions) followed by Western blot. Inter-alpha-trypsin immunoreactive proteins were detected by enhanced chemiluminescence. Stone formation was confirmed to be active radiologically or passed as stone or gravel within 12 months of the sample. Stone composition was confirmed crystallographically. Normal individuals had no personal or familial history of urolithiasis and matched stone forming patients regarding race (white) and age (23 to 71 years old). Urine from a total of 101 individuals was analyzed. The intact inter-alpha-trypsin trimer (approximately 220 to 240 kDa.) and heavy chain (HC) 2-bikunin/HC1-bikunin dimers (approximately 115 to 130 kDa.) were detected more often in stone forming men (23 of 26 [89%] and 26 of 26 [100%], respectively) than in normal individuals (6 of 26 [23%] and 5 of 26 [19%], respectively, p < 0.0001). In those normal in...Continue Reading

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Citations

Aug 26, 1999·In Vitro Cellular & Developmental Biology. Animal·R M SramkoskiJ W Jacobberger
Oct 4, 2005·The Journal of Clinical Investigation·Fredric L CoeElaine Worcester
Nov 11, 2008·Connective Tissue Research·Lisheng Zhuo, Koji Kimata
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Jul 7, 2010·Scandinavian Journal of Urology and Nephrology·Mehri IgciA Tuncay Demiryurek
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May 8, 1999·Journal of the American Society of Nephrology : JASN·S IidaS R Khan
Jan 3, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·R C HedgepethS R Marengo

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