Differential Expression of VGLUT2 in Mouse Mesopontine Cholinergic Neurons

ENeuro
Thomas SteinkellnerThomas S Hnasko

Abstract

Vesicular glutamate transporters (VGLUTs) mediate the synaptic uptake of glutamate from the cytosol into synaptic vesicles and are considered unambiguous neurochemical markers of glutamate neurons. However, many neurons not classically thought of as glutamatergic also express a VGLUT and co-release glutamate. Using a genetic fate-mapping strategy we found that most cholinergic neurons in the mouse mesopontine tegmentum express VGLUT2 at some point during development, including the pedunculopontine tegmental nucleus (PPTg), laterodorsal tegmental nucleus, and parabigeminal nucleus (PBG), but not the oculomotor nucleus. In contrast, very few of these cholinergic neurons displayed evidence of vesicular GABA transporter expression. Using multiplex fluorescent in situ hybridization, we determined that only PBG cholinergic neurons are also predominantly positive for VGLUT2 mRNA in the adult, with only small numbers of PPTg cholinergic neurons overlapping with VGLUT2 mRNA. Using Cre-dependent viral vectors we confirm these in situ hybridization data, and demonstrate projection patterns of cholinergic and glutamatergic populations. These results demonstrate that most mesopontine cholinergic neurons may transiently express VGLUT2, but t...Continue Reading

Citations

Sep 2, 2020·The Journal of Comparative Neurology·Maxime FougèreDimitri Ryczko
Feb 2, 2021·ACS Chemical Neuroscience·Thomas SteinkellnerDaniela Boassa
Apr 27, 2021·Frontiers in Neural Circuits·Cornelis Immanuel van der ZouwenDimitri Ryczko
Aug 28, 2021·The Journal of Comparative Neurology·Guela SokhadzeWilliam Guido

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