Differential Expression of Virulence Genes and Motility in Ralstonia (Pseudomonas) solanacearum during Exponential Growth.

Applied and Environmental Microbiology
S J CloughT P Denny

Abstract

A complex network regulates virulence in Ralstonia solanacearum (formerly Pseudomonas solanacearum); central to this system is PhcA, a LysR-type transcriptional regulator. We report here that two PhcA-regulated virulence factors, endoglucanase (Egl) and acidic exopolysaccharide I (EPS I), and motility are expressed differentially during exponential growth in batch cultures. Tests with strains carrying lacZ fusions in a wild-type genetic background revealed that expression (on a per-cell basis) of phcA was constant but expression of egl and epsB increased 20- to 50-fold during multiplication from 1 x 10(sup7) to 5 x 10(sup8) CFU/ml. Expression of xpsR, an intermediate regulator downstream of PhcA in the regulatory cascade for eps expression, was similar to that of epsB and egl. Motility track photography revealed that all strains were essentially nonmotile at 10(sup6) CFU/ml. As cell density increased, 30 to 50% of wild-type cells were motile between 10(sup7) and 10(sup8) CFU/ml, but this population was again nonmotile at 10(sup9) CFU/ml. In contrast, about 60% of the cells of phcB and phcA mutants remained motile at 10(sup9) CFU/ml. Expression of phcB, which is not positively regulated by PhcA, was the inverse of epsB, egl, and...Continue Reading

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Citations

Aug 29, 2001·FEMS Microbiology Reviews·N A WhiteheadG P Salmond
May 1, 2000·Molecular Plant Pathology·J Tans-KerstenC Allen
May 6, 2003·Annual Review of Phytopathology·Susanne B Von BodmanDavid L Coplin
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Apr 15, 2017·Critical Reviews in Microbiology·Joachim VandecraenRob Van Houdt
Jul 6, 2019·Applied Microbiology and Biotechnology·Siel DesmetEmmy Dhooghe
May 24, 2001·Journal of Bacteriology·J Tans-KerstenC Allen
Mar 23, 2011·Journal of Bacteriology·Fanhong MengCaitilyn Allen
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May 9, 2012·Annual Review of Phytopathology·Stéphane Genin, Timothy P Denny
Jun 1, 2011·World Journal of Microbiology & Biotechnology·Saeed Tarighi, Parissa Taheri

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