Differential expression of vvhA and CPS operon allele 1 genes in Vibrio vulnificus under biofilm and planktonic conditions.

Antonie van Leeuwenhoek
Sedat Çam, Robin Brinkmeyer

Abstract

Examination of genes encoding for the virulence factors, hemolysin/cytolysin (vvhA) and capsular polysaccharide (CPS allele 1), during biofilm formation revealed that their expression was influenced by the maturity of the biofilm as well as by temperature. At 24 °C, expression of vvhA during biofilm formation was low between 4 and 12 h but increased 10-fold by 24 h to (5.1 × 104 ± 6.3 × 103mRNA copies/ml) as the biofilm matured. Compared to planktonic cells, expression of vvhA during biofilm formation at 24 °C was initially up-regulated at 4 h (1.07 ± 0.00-fold) but then was down-regulated almost four-fold during the intermediate and mature stages of biofilm formation. In contrast, vvhA expression at 37 °C was up-regulated almost four-fold in the early stages (4 and 6 h) of biofilm formation and remained two-fold up-regulated by 24 h even as the biofilm was deteriorating. CPS allele 1 expression at 24 °C during biofilm formation was up-regulated (1.50 ± 0.18-fold) during the initial attachment phase of the cells but was strongly down-regulated during the intermediate phases at 8 and 10 h (74.42 ± 42.16-fold and 453.76 ± 193.32-fold, respectively), indicating that capsular polysaccharide (CPS) is not important to intermediate bi...Continue Reading

References

Jan 1, 1987·Annual Review of Microbiology·J W CostertonT J Marrie
Feb 1, 1988·Archives of Surgery·R J Howard, S Lieb
Feb 1, 1987·The Journal of Infectious Diseases·L D Gray, A S Kreger
Aug 1, 1984·Infection and Immunity·S D HelmsJ C Travis
Jul 1, 1995·Applied and Environmental Microbiology·J D Oliver, R Bockian
Jan 1, 1993·Life Sciences·H R KimS D Park
Sep 1, 1993·The Journal of Infectious Diseases·U HayatJ G Morris
Jan 1, 1996·Annual Review of Microbiology·I S Roberts
Feb 13, 1999·European Journal of Pharmacology·H KookY H Baik
May 4, 1999·Infection and Immunity·A C WrightM B Sztein
May 26, 1999·FEMS Microbiology Letters·D A Linkous, J D Oliver
Oct 8, 1999·Emerging Infectious Diseases·P S MeadR V Tauxe
Apr 1, 2000·Microbes and Infection·M S Strom, R N Paranjpye
Jan 3, 2001·Journal of Bacteriology·D McDougaldS Kjelleberg
Dec 9, 2003·Applied and Environmental Microbiology·Mark S Campbell, Anita C Wright
Jan 20, 2004·Journal of Bacteriology·Lavin A Joseph, Anita C Wright
Jul 17, 2004·Molecular Microbiology·Anna D Tischler, Andrew Camilli
Jul 24, 2004·Infection and Immunity·Jong-Ho LeeSoon-Jung Park
Aug 20, 2004·Journal of Bacteriology·Yasuhiko IrieMing H Yuk
May 5, 2005·Applied and Environmental Microbiology·Alexandra ReschFriedrich Götz
Jun 21, 2005·Epidemiology and Infection·J D Oliver
Feb 18, 2006·Journal of Bacteriology·Maria Chatzidaki-LivanisAnita C Wright
May 8, 2007·Annual Review of Microbiology·Rita TamayoAndrew Camilli
Feb 24, 2009·Trends in Microbiology·Fitnat H Yildiz, Karen L Visick
Mar 4, 2009·Infection and Immunity·Melissa K Jones, James D Oliver
Jun 3, 2010·Infection and Immunity·Rita TamayoAndrew Camilli
Jun 3, 2015·ACS Applied Materials & Interfaces·Huabin WangMichelle L Gee

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Citations

Nov 12, 2021·Frontiers in Microbiology·Karlen Enid Correa Velez, Robert Sean Norman

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Methods Mentioned

BETA
PCR

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