Differential expression patterns of recombination-activating genes in individual mature B cells in juvenile idiopathic arthritis

Annals of the Rheumatic Diseases
C FaberHermann J Girschick

Abstract

Re-expression of the recombination-activating genes (RAG) in peripheral B cells may be relevant in the development of autoreactive antibodies in autoimmune diseases. The presence of antinuclear antibodies (ANA) as a hallmark of oligoarticular juvenile idiopathic arthritis (o-JIA, early-onset type) indicates a breakdown in immunological tolerance. To examine the expression of RAG genes in peripheral blood mature B lymphocytes in patients with o-JIA. 777 memory B cells from peripheral blood, CD19+ CD27+ CD5+ or CD19+ CD27+ CD5-, isolated from three ANA+ children with o-JIA and three healthy age-matched children, were examined for the expression of RAG1 and RAG2 mRNA. mRNA transcripts of activation-induced cytidine deaminase and immunoglobulin G were searched to further determine their developmental stage. mRNA was present for any of the two RAG genes in the B cells of children with JIA and controls. However, the predominance of RAG1 or RAG2 was different. A significantly decreased frequency of RAG2-expressing memory B cells in both CD5+ and CD5- populations was noted in children with JIA (p<0.001), whereas the number of RAG1-expressing B cells was slightly increased. The coordinate expression of both the RAG genes was a rare even...Continue Reading

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Citations

Mar 1, 2008·Zeitschrift für Rheumatologie·T NiehuesT Telieps
Jan 1, 2010·Arthritis·V WiegeringH Morbach
Aug 27, 2009·Biologics : Targets & Therapy·Jasmin B Kuemmerle-Deschner, Sm Benseler
Apr 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lynda MazariMoncef Zouali
May 21, 2013·FEBS Letters·Moncef Zouali
Jun 21, 2011·Lancet·Berent PrakkenAlberto Martini
Feb 9, 2008·Trends in Immunology·Moncef Zouali
Jan 22, 2011·Annals of the New York Academy of Sciences·Moncef Zouali
Aug 7, 2007·Molecular Immunology·P RichlH J Girschick
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hui-Chen HsuJohn D Mountz
Oct 9, 2009·Autoimmunity·H Morbach, H J Girschick
Mar 7, 2019·Frontiers in Immunology·Meredyth G Ll Wilkinson, Elizabeth C Rosser

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