Differential extraction and protein sequencing reveals major differences in patterns of primary cell wall proteins from plants.

The Journal of Biological Chemistry
D RobertsonA R Slabas

Abstract

The proteins of the primary cell walls of suspension cultured cells of five plant species, Arabidopsis, carrot, French bean, tomato, and tobacco, have been compared. The approach that has been adopted is differential extraction followed by SDS-polyacrylamide gel electrophoresis (PAGE), rather than two-dimensional gel analysis, to facilitate protein sequencing. Whole cells were washed sequentially with the following aqueous solutions, CaCl2, CDTA (cyclohexane diaminotetraacetic acid, DTT (dithiothreitol), NaCl, and borate. SDS-PAGE analysis showed consistent differences between species. From the 233 proteins that were selected for sequencing, 63% gave N-terminal data. This analysis shows that (i) patterns of proteins revealed by SDS-PAGE are strikingly different for all five species, (ii) a large number of these proteins cannot be identified by data base searches indicating that a significant proportion of wall proteins have not been previously described, (iii) the major proteins that can be identified belong to very different classes of proteins, (iv) the majority of proteins found in the extracellular growth media are absent from their respective cell wall extracts, and (v) the results of the extraction process are indicative ...Continue Reading

References

Oct 1, 1990·Current Opinion in Cell Biology·K Roberts
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 1, 1994·Electrophoresis·A TsugitaY Nozu
Feb 1, 1994·The Plant Journal : for Cell and Molecular Biology·M J Kieliszewski, D T Lamport
Jan 1, 1993·The Plant Cell·A M Showalter
Jan 1, 1996·The Plant Journal : for Cell and Molecular Biology·R CookeH Höfte
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y ElkindC J Lamb
Apr 1, 1993·Plant Physiology·B. Keller

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Citations

Mar 6, 1999·Current Opinion in Plant Biology·R Pennell
Feb 18, 2004·Plant Physiology·Patricia M LonoskySteve R Rodermel
Jul 14, 2012·Plant Physiology·Zhang YangBent L Petersen
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·I AllonaR W Whetten
Mar 18, 2009·Cellular and Molecular Life Sciences : CMLS·Anne BernaAndrew Suh
Jan 26, 2016·Journal of Proteomics·Xin Wang, Setsuko Komatsu
Nov 26, 2008·Plant Physiology and Biochemistry : PPB·Elizabeth J DavisYajun Wu
Aug 19, 2004·The Plant Journal : for Cell and Molecular Biology·Jocelyn K C RoseRamu Subramanian Saravanan
Apr 11, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Anne BernaFrançois Bernier
Feb 20, 2009·Physiologia Plantarum·Won Kyong ChoJae-Yean Kim
Nov 3, 2010·Mass Spectrometry Reviews·Ganesh Kumar AgrawalRandeep Rakwal
Jan 3, 2013·Proteomics·Arnaud DayCaroline Tokarski
Jan 18, 2014·Proteomics·Maria Juliana Calderan-RodriguesCarlos Alberto Labate
Feb 15, 2005·Plant Physiology and Biochemistry : PPB·Geneviève EphritikhineNorbert Rolland
Feb 15, 2005·Plant Physiology and Biochemistry : PPB·Sang-Jik LeeJocelyn K C Rose
Jul 28, 2004·Phytochemistry·Bonnie S WatsonLloyd W Sumner
May 12, 2004·Current Opinion in Plant Biology·Jocelyn K C RoseCarmen Catalá
Dec 17, 2005·Trends in Plant Science·Elisabeth JametRafael F Pont-Lezica
Jul 22, 2015·Molecules and Cells·Won Kyong ChoJae-Yean Kim
May 12, 2007·Microbiological Research·Vijai BhadauriaYou-Liang Peng
Jul 1, 2010·Journal of Biomedicine & Biotechnology·Raquel González-FernándezJesús V Jorrín-Novo
Sep 27, 2006·Molecular & Cellular Proteomics : MCP·Jean Paul BrizardChristophe Brugidou
Sep 15, 2006·Proteomics·Sixue Chen, Alice C Harmon
Jan 23, 2008·Proteomics·Elisabeth JametRafael Pont-Lezica

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