Differential fadE28 expression associated with phenotypic virulence of Mycobacterium tuberculosis

Microbial Pathogenesis
T H J LamW C Yam

Abstract

Ability to persist in human macrophages is central to the virulence of Mycobacterium tuberculosis and is not invariable among various strains. Differential gene expression that is associated with phenotypic virulence may provide additional information of virulent genes involved in the pathogenesis of M. tuberculosis, which is not fully elucidated. Three hypervirulent strains of M. tuberculosis isolated from patients suffering with tuberculous meningitis were shown to grow more rapidly inside human macrophages in a previous study. In the current investigation, expression of 7 mycobacterial genes (fadE28, mce1A, mymA, acr, sigA, sugC, and Rv3723) of these strains during ex vivo macrophage challenge and in vitro acid shock was quantified by real-time PCR. Using rrs gene as a normalisation gene, fadE28 gene exhibited differential gene expression that is associated with phenotypic virulence, whereas the other 6 genes showed indistinguishable expression patterns. Up-regulation of fadE28 gene in the hypervirulent strains may account for virulence by increasing the efficiency of beta-oxidation, which is important for the persistence in macrophages as M. tuberculosis uses fatty acids preferably inside phagosome of macrophages. The fadE2...Continue Reading

References

Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D M CollinsW R Jacobs
Apr 16, 1998·Biochemical and Biophysical Research Communications·U Mahadevan, G Padmanaban
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y YuanC E Barry
Apr 7, 1999·The Journal of Infectious Diseases·M ZhangP F Barnes
Sep 9, 2000·FEMS Microbiology Reviews·M BraibantJ Content
Sep 10, 2002·Journal of Bacteriology·R D FleischmannC M Fraser
Dec 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lanbo ShiRobert J North
Sep 4, 2003·The Journal of Experimental Medicine·Dirk SchnappingerGary K Schoolnik
Jan 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·W SEGAL
Oct 22, 2003·FEMS Microbiology Letters·Amit SinghAnil K Tyagi
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christopher M Sassetti, Eric J Rubin
Jan 20, 2004·European Journal of Biochemistry·Sandro Ghisla, Colin Thorpe
Aug 1, 1952·The Journal of Experimental Medicine·E SUTER
Sep 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kevin PetheDavid G Russell
Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jyothi RengarajanEric J Rubin
Dec 22, 2005·Cellular Microbiology·Ernesto J Muñoz-Elías, John D McKinney

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Citations

Mar 3, 2011·Microbial Pathogenesis·J T LamW C Yam
Apr 1, 2012·Environmental Microbiology Reports·Iria UhíaJosé L García
Jun 18, 2010·The FEBS Journal·Laxman S Meena, Rajni
Mar 9, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases· Rajni, Laxman S Meena
Jul 28, 2017·Veterinary Pathology·Amanda J Martinot
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Noton K Dutta, Petros C Karakousis

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