Differential fate of multipotent and lineage-restricted neural precursors following transplantation into the adult CNS

Neuron Glia Biology
A C LeporeItzhak Fischer

Abstract

Multiple classes of precursor cells have been isolated and characterized from the developing spinal cord including multipotent neuroepithelial (NEP) stem cells and lineage-restricted precursors for neurons (NRPs) and glia (GRPs). We have compared the survival, differentiation and integration of multipotent NEP cells with lineage-restricted NRPs and GRPs using cells isolated from transgenic rats that express the human placental alkaline phosphatase gene. Our results demonstrate that grafted NEP cells survive poorly, with no cells observed 3 days after transplant in the adult hippocampus, striatum and spinal cord, indicating that most CNS regions are not compatible with transplants of multipotent cells derived from fetal CNS. By contrast, at 3 weeks and 5 weeks post-engraftment, lineage-restricted precursors showed selective migration along white-matter tracts and robust survival in all three CNS regions. The grafted precursors expressed the mature neuronal markers NeuN and MAP2, the astrocytic marker GFAP, the oligodendrocytic markers RIP, NG2 and Sox-10, and the synaptic marker synaptophysin. Similar behavior was observed when these precursors were transplanted into the injured spinal cord. Predifferentiated, multipotent NEP ce...Continue Reading

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Citations

Jan 28, 2010·Journal of Neurotrauma·Jill SeeItzhak Fischer
Feb 8, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Tim MagnusMahendra S Rao
May 16, 2007·Journal of Neuroengineering and Rehabilitation·Ali Samadikuchaksaraei
Oct 23, 2008·Journal of Neurosurgery. Spine·Birgit NeuhuberItzhak Fischer
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Nov 13, 2009·Journal of Neuroscience Research·Joseph F BonnerItzhak Fischer
Jan 17, 2015·Brain Research·Joseph F Bonner, Oswald Steward
May 24, 2011·Journal of Neuroscience Methods·Ying JinItzhak Fischer
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May 24, 2016·Neural Regeneration Research·Kazuo HayakawaItzhak Fischer
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