Differential filamentation of Candida albicans and Candida dubliniensis Is governed by nutrient regulation of UME6 expression.

Eukaryotic Cell
Leanne O'ConnorGary P Moran

Abstract

Candida dubliniensis is closely related to Candida albicans; however, it is responsible for fewer infections in humans and is less virulent in animal models of infection. C. dubliniensis forms fewer hyphae in vivo, and this may contribute to its reduced virulence. In this study we show that, unlike C. albicans, C. dubliniensis fails to form hyphae in yeast extract-peptone-dextrose (YPD) medium supplemented with 10% (vol/vol) fetal calf serum (YPDS medium). However, C. dubliniensis filaments in water plus 10% (vol/vol) fetal calf serum (WS), and this filamentation is inhibited by the addition of peptone and glucose. Repression of filamentation in YPDS medium could be partly overcome by preculture in synthetic Lee's medium. Unlike C. albicans, inoculation of C. dubliniensis in YPDS medium did not result in increased UME6 transcription. However, >100-fold induction of UME6 was observed when C. dubliniensis was inoculated in nutrient-poor WS medium. The addition of increasing concentrations of peptone to WS medium had a dose-dependent effect on reducing UME6 expression. Transcript profiling of C. dubliniensis hyphae in WS medium identified a starvation response involving expression of genes in the glyoxylate cycle and fatty acid ox...Continue Reading

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Citations

Jun 16, 2012·Clinical Oral Investigations·C KragelundE P Bennett
May 28, 2013·Eukaryotic Cell·Uttara ChakrabortyKaustuv Sanyal
Nov 16, 2010·Eukaryotic Cell·Gary P MoranDerek J Sullivan
Jul 19, 2011·Eukaryotic Cell·Delma S ThompsonDavid Kadosh
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Jun 25, 2019·Frontiers in Microbiology·Ruvini U PathiranaMira Edgerton
May 10, 2017·BMC Complementary and Alternative Medicine·Sameh S M SolimanAshraf S Ibrahim

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