Differential gel electrophoresis of the tear proteome

Optometry and Vision Science : Official Publication of the American Academy of Optometry
Maria MarkoulliBrien Holden

Abstract

This study evaluates the use of differential gel electrophoresis (DIGE) for tear analysis and applies this technique to establish the effect of extended contact lens wear on the tear proteome. Flush tears were collected from nine healthy non-contact lens wearers at baseline, during the first day, and after 1 month of contact lens wear. Participants wore AIR OPTIX on an extended wear (EW) schedule. Tears were collected at mid-day and upon waking and analyzed for concentrations of total protein using the bicinchoninic acid assay method. DIGE was then performed to detect biomarkers likely to be affected by contact lens wear. Technique variability, physiological variability, and the effect of EW were established. Proteins with significantly changed abundance during lens wear were cut and identified with mass spectrometry. Both Cy3 and Cy5 identified the same proteins relative to Cy2 with the highest fold difference of 4.4. The proteome varied by 3% at the same time point, with a significant increase in protein spots found between mid-day (257) and upon awakening (298). With EW, of the 311 proteins spots identified upon awakening, 15 differed significantly. Of the five differences between baseline and the first night of EW, all were...Continue Reading

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Citations

Jul 25, 2013·Experimental Eye Research·Nadine von Thun Und Hohenstein-BlaulFranz H Grus
Jun 12, 2013·Proteomics·Richard D SembaJennifer E Van Eyk
Dec 30, 2016·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Maria MarkoulliJingjing You
Aug 5, 2017·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Maureen BoostZhaoran Wang
Jun 13, 2020·Frontiers in Veterinary Science·Ana Cláudia RaposoArianne Pontes Oriá

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