Differential gene expression and alternative splicing in insect immune specificity

BMC Genomics
C E RiddellEamonn B Mallon

Abstract

Ecological studies routinely show genotype-genotype interactions between insects and their parasites. The mechanisms behind these interactions are not clearly understood. Using the bumblebee Bombus terrestris/trypanosome Crithidia bombi model system (two bumblebee colonies by two Crithidia strains), we have carried out a transcriptome-wide analysis of gene expression and alternative splicing in bees during C. bombi infection. We have performed four analyses, 1) comparing gene expression in infected and non-infected bees 24 hours after infection by Crithidia bombi, 2) comparing expression at 24 and 48 hours after C. bombi infection, 3) determining the differential gene expression associated with the bumblebee-Crithidia genotype-genotype interaction at 24 hours after infection and 4) determining the alternative splicing associated with the bumblebee-Crithidia genotype-genotype interaction at 24 hours post infection. We found a large number of genes differentially regulated related to numerous canonical immune pathways. These genes include receptors, signaling pathways and effectors. We discovered a possible interaction between the peritrophic membrane and the insect immune system in defense against Crithidia. Most interestingly, ...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1997·Molecular and Cellular Neurosciences·T P GschwendP Sonderegger
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·A DanielliFotis C Kafatos
Jul 25, 2000·Insect Molecular Biology·M T FerdigB M Christensen
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·E De GregorioBruno Lemaitre
Jan 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Evy VierstraeteLiliane Schoofs
Jan 1, 1997·Annual Review of Entomology·M J Lehane
Oct 20, 2004·Journal of Invertebrate Pathology·P Korner, Paul Schmid-Hempel
Aug 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yasuyuki ArakaneKarl J Kramer
Aug 20, 2005·Science·Fiona L WatsonDietmar Schmucker
Sep 16, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Paul Schmid-Hempel
Jan 5, 2007·Annual Review of Immunology·Bruno Lemaitre, Jules Hoffmann
May 19, 2007·Developmental and Comparative Immunology·Christine LeschUlrich Theopold
Jul 17, 2007·Archives of Insect Biochemistry and Physiology·Bieke ScharlakenFrans J Jacobs
Oct 16, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Chris Hauton, Valerie J Smith
Nov 27, 2007·Developmental and Comparative Immunology·Lindsey S GarverGeorge Dimopoulos
Dec 7, 2007·Current Biology : CB·Pascale F Dijkers, Patrick H O'Farrell
Dec 11, 2007·The Journal of Biological Chemistry·Alvaro Molina-CruzCarolina Barillas-Mury
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Nov 29, 2008·Insect Biochemistry and Molecular Biology·R R DinglasanM Jacobs-Lorena
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Jun 10, 2009·Bioinformatics·Heng Li1000 Genome Project Data Processing Subgroup
Jun 27, 2009·PLoS Genetics·Sandra Fausia SoukupDavid Gubb
Dec 23, 2009·Developmental and Comparative Immunology·Chris M CirimotichGeorge Dimopoulos
Feb 12, 2010·Bioinformatics·Thomas D Wu, Serban Nacu
Feb 25, 2010·Genome Biology·Nicole M GerardoAndreas Vilcinskas
Jun 24, 2010·Journal of Cell Science·Anna WäheMaximiliano G Gutierrez
Nov 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jianming ChenJiahuai Han
Jul 27, 2011·PloS One·Fran SupekTomislav Šmuc
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Takayuki KuraishiBruno Lemaitre
Jan 24, 2012·Biochemical and Biophysical Research Communications·Katerina Karkali, George Panayotou
Feb 14, 2012·Trends in Immunology·Hyota Takamatsu, Atsushi Kumanogoh
Jun 23, 2012·Genome Research·Simon AndersWolfgang Huber
Jul 19, 2013·Biochemical Society Transactions·Olga MayansDaniel J Rigden
Sep 26, 2013·Developmental and Comparative Immunology·Soni Deshwal, Eamonn B Mallon
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Seth M BarribeauPaul Schmid-Hempel

Citations

Jul 17, 2016·Zoology : Analysis of Complex Systems, ZACS·Jenny M GreenwoodLudovic Mallet
Jan 28, 2018·Applied and Environmental Microbiology·Blair K MocklerHauke Koch
May 14, 2019·Frontiers in Cellular and Infection Microbiology·Qiushi LiuTatsuhiko Kadowaki
Dec 14, 2017·Memórias do Instituto Oswaldo Cruz·João Ramalho Ortigão-FariasYara Maria Traub-Csekö
Sep 25, 2020·Royal Society Open Science·Erin L ColeRebeca B Rosengaus

Related Concepts

Metazoa
Crithidia
Host-Parasite Interactions
Insecta
Disease Clustering
Nested Transcripts
Computational Molecular Biology
MRNA Differential Display
Serine Proteases
Alternative Splicing

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