Differential gene expression by integrin beta 7+ and beta 7- memory T helper cells

BMC Immunology
Madeleine W RodriguezD J Erle

Abstract

The cell adhesion molecule integrin alpha 4 beta 7 helps direct the migration of blood lymphocytes to the intestine and associated lymphoid tissues. We hypothesized that beta 7+ and beta 7- blood memory T helper cells differ in their expression of genes that play a role in the adhesion or migration of T cells. RNA was prepared from beta 7+ and beta 7- CD4+ CD45RA- blood T cells from nine normal human subjects and analyzed using oligonucleotide microarrays. Of 21357 genes represented on the arrays, 16 were more highly expressed in beta 7+ cells and 18 were more highly expressed in beta 7- cells (>/=1.5 fold difference and adjusted P < 0.05). Several of the differentially expressed transcripts encode proteins with established or putative roles in lymphocyte adhesion and chemotaxis, including the chemokine receptors CCR9 and CCR10, the integrin alpha 4 subunit, L-selectin, KLRB1 (CD161), NT5E (CD73), LGALS1 and LGALS2 (galectin-1 and -2), and RGS1. Flow cytometry was used to determine whether differences in levels of transcripts encoding cell surface proteins were associated with differential expression of those proteins. Using this approach, we found that surface expression of KLRB1, LAIR1, and NT5E proteins was higher on beta 7+...Continue Reading

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Citations

Jan 30, 2007·Journal of Translational Medicine·Abel TorresWoubalem Birmachu
Feb 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Melinda RákiLudvig M Sollid
Sep 9, 2006·Environmental Microbiology·Dawn E HolmesDerek R Lovley
Aug 9, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Navin Kumar Verma, Dermot Kelleher
Jan 27, 2018·Digestive Diseases and Sciences·Elisa K BodenJames D Lord
Jan 26, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·John W NorthfieldPaul Klenerman
Mar 1, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alice MilesDavid H Adams
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Aug 3, 2007·Journal of Molecular Biology·Koen BartholomeeusenFrauke Christ

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Datasets Mentioned

BETA
AB020640
-1.9
GSE1039

Methods Mentioned

BETA
flow cytometry
GTPase
FACS

Software Mentioned

GenePix
Operon
limma
MIAME
Bioconductor package
Bioconductor
R
marrayNorm

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