Differential Gene Expression Caused by the F and M Loci Provides Insight Into Ethylene-Mediated Female Flower Differentiation in Cucumber

Frontiers in Plant Science
Jian PanRun Cai

Abstract

In cucumber (Cucumis sativus L.), the differentiation and development of female flowers are important processes that directly affect the fruit yield and quality. Sex differentiation is mainly controlled by three ethylene synthase genes, F (CsACS1G), M (CsACS2), and A (CsACS11). Thus, ethylene plays a key role in the sex differentiation in cucumber. The "one-hormone hypothesis" posits that F and M regulate the ethylene levels and initiate female flower development in cucumber. Nonetheless, the precise molecular mechanism of this process remains elusive. To investigate the mechanism by which F and M regulate the sex phenotype, three cucumber near-isogenic lines, namely H34 (FFmmAA, hermaphroditic), G12 (FFMMAA, gynoecious), and M12 (ffMMAA, monoecious), with different F and M loci were generated. The transcriptomic analysis of the apical shoots revealed that the expression of the B-class floral homeotic genes, CsPI (Csa4G358770) and CsAP3 (Csa3G865440), was immensely suppressed in G12 (100% female flowers) but highly expressed in M12 (∼90% male flowers). In contrast, CAG2 (Csa1G467100), which is an AG-like C-class floral homeotic gene, was specifically highly expressed in G12. Thus, the initiation of female flowers is likely to b...Continue Reading

References

Dec 20, 1968·Science·A L McMurray, C H Miller
Aug 22, 1997·Science·S E Jacobsen, E M Meyerowitz
Dec 23, 1999·Plant Molecular Biology·A KahanaR Perl-Treves
Oct 19, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·H Mibus, T Tatlioglu
Jul 17, 2007·Journal of Experimental Botany·Sayoko SaitoHideyuki Takahashi
Sep 15, 2007·Development·Kristina GremskiMartin F Yanofsky
Aug 21, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zheng LiRun Cai
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Mar 25, 2009·The Plant Journal : for Cell and Molecular Biology·Andrea ChiniRoberto Solano
Nov 3, 2009·Nature Genetics·Sanwen HuangSonggang Li
Dec 25, 2009·The Plant Journal : for Cell and Molecular Biology·Dong-Hui WangShu-Nong Bai
Sep 10, 2011·Genome Research·Sonia TarazonaAna Conesa
Jul 30, 2014·Current Opinion in Plant Biology·Susheng SongDaoxin Xie
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Sep 10, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Junyi TanZheng Li
Nov 7, 2015·Science·Adnane BoualemAbdelhafid Bendahmane
Jul 13, 2016·Molecular Plant·Huiming ChenSanwen Huang
Aug 20, 2016·Frontiers in Plant Science·Jin-Jing SunShu-Nong Bai
Nov 30, 2016·Clinical Epigenetics·Tobias FehlmannAndreas Keller
Jan 20, 2017·Molecular Ecology·Jos KäferJohn R Pannell

❮ Previous
Next ❯

Citations

Sep 19, 2019·The Plant Journal : for Cell and Molecular Biology·Jie ZhangYong Xu
Oct 28, 2019·Frontiers in Plant Science·Dandan LiZheng Li
Oct 24, 2020·Journal of Integrative Plant Biology·He ZhaoJin-Song Zhang
Feb 1, 2021·Current Opinion in Plant Biology·Cecilia Martínez, Manuel Jamilena
Sep 21, 2021·Frontiers in Plant Science·Qingfei LiXinzheng Li

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJEB25272
SAMEA3469515

Methods Mentioned

BETA
RNA-Seq
chemical treatments
One-Hybrid
transgenic

Software Mentioned

Primer
BLASTP
OmicShare
SAS
Venny
Bowtie2
Venn
STRING

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.