Differential gene expression during sporulation in Bacillus subtilis: structure and regulation of the spoIIID gene

Molecular Microbiology
C M Stevens, J Errington

Abstract

The gene spoIIID, which is essential for spore formation in Bacillus subtilis, was cloned and sequenced. It consists of one open reading frame which would encode a 93-amino-acid protein with a classic helix-turn-helix motif, characteristic of sequence-specific DNA-binding proteins. SpoIIID protein is a previously identified transcription factor, capable of altering the specificity of RNA polymerase containing sigma K in vitro (Kroos et al., 1989). The spoIIID83 mutation (by which the locus was originally identified), was sequenced and found to be a single base substitution in the ribosome binding site upstream of the spoIIID open reading frame. A transcriptional fusion to lacZ was constructed and used to examine the regulation of spoIIID. Expression of spoIIID occurred only during sporulation, beginning 1.5 to 2 hours after the initiation of sporulation. The dependence of spoIIID expression on other spo loci suggests that it is mother-cell-specific, and that it is transcribed by sigma E-containing RNA polymerase.

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Citations

Mar 29, 2003·Journal of Molecular Biology·Patrick EichenbergerRichard Losick
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A Driks, R Losick
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