Differential gene expression in classic giant cell tumours of bone: Tenascin C as biological risk factor for local relapses and metastases

Histopathology
L PazzagliaM S Benassi

Abstract

To identify candidate prognostic biological markers useful in selecting high-risk patients with classic primary giant cell tumours (GCT). GCT specimens with different behaviour associated with an increased risk of local and/or distant relapses were studied. Screening mRNA microarray analysis followed by real-time polymerase chain reaction and immunohistochemistry on tissue microarray sections was used to validate the prognostic role of differentially expressed genes on a larger series by statistical analysis tests. Global gene expression profiling identified 109 differentially expressed genes according to prognosis. A correlation was found between mRNA levels and clinical outcome identifying Tenascin C (TNC) as the most significant prognostic biological marker. By means of backward Wald logistic regression, TNC overexpression defined an eightfold increased risk for metastasis and fourfold for local recurrence. At the protein level, TNC immunoreactivity resulted in a significant difference in the disease-free survival probability curves, providing a stratification for GCT patients, useful for predicting relapse. Our study provides important data for the selection of biomarkers with a significant clinical impact and suggests the ...Continue Reading

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Citations

Aug 6, 2011·Cellular and Molecular Life Sciences : CMLS·Kim S MidwoodGertraud Orend
Mar 13, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Irene QuattriniMaria Serena Benassi
May 15, 2013·Research in Veterinary Science·Leonardo LeonardiMaria Serena Benassi
Jun 23, 2011·Journal of Cellular and Molecular Medicine·Florence Brellier, Ruth Chiquet-Ehrismann
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