Differential gene expression in dentate granule cells in mesial temporal lobe epilepsy with and without hippocampal sclerosis

Epilepsia
Nicole G GriffinErin L Heinzen

Abstract

Hippocampal sclerosis is the most common neuropathologic finding in cases of medically intractable mesial temporal lobe epilepsy. In this study, we analyzed the gene expression profiles of dentate granule cells of patients with mesial temporal lobe epilepsy with and without hippocampal sclerosis to show that next-generation sequencing methods can produce interpretable genomic data from RNA collected from small homogenous cell populations, and to shed light on the transcriptional changes associated with hippocampal sclerosis. RNA was extracted, and complementary DNA (cDNA) was prepared and amplified from dentate granule cells that had been harvested by laser capture microdissection from surgically resected hippocampi from patients with mesial temporal lobe epilepsy with and without hippocampal sclerosis. Sequencing libraries were sequenced, and the resulting sequencing reads were aligned to the reference genome. Differential expression analysis was used to ascertain expression differences between patients with and without hippocampal sclerosis. Greater than 90% of the RNA-Seq reads aligned to the reference. There was high concordance between transcriptional profiles obtained for duplicate samples. Principal component analysis re...Continue Reading

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Apr 14, 2015·Epilepsia·Aleksandar J RistićIvan Spasojević

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Citations

Mar 3, 2017·Genome Research·Prashant Kumar SrivastavaMichael R Johnson
Jul 2, 2019·Experimental and Therapeutic Medicine·Yan Wang, Zhiping Li
Nov 7, 2019·Epilepsy & Behavior : E&B·José Ramón BosqueDolores E López
Jun 24, 2021·Brain Communications·Geoffrey PiresOrrin Devinsky

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