Differential gene expression in individual papilla-resistant and powdery mildew-infected barley epidermal cells

Molecular Plant-microbe Interactions : MPMI
Torben GjettingMichael F Lyngkjaer

Abstract

Resistance and susceptibility in barley to the powdery mildew fungus (Blumeria graminis f. sp. hordei) is determined at the single-cell level. Even in genetically compatible interactions, attacked plant epidermal cells defend themselves against attempted fungal penetration by localized responses leading to papilla deposition and reinforcement of their cell wall. This conveys a race-nonspecific form of resistance. However, this defense is not complete, and a proportion of penetration attempts succeed in infection. The resultant mixture of infected and uninfected leaf cells makes it impossible to relate powdery mildew-induced gene expression in whole leaves or even dissected epidermal tissues to resistance or susceptibility. A method for generating transcript profiles from individual barley epidermal cells was established and proven useful for analyzing resistant and successfully infected cells separately. Contents of single epidermal cells (resistant, infected, and unattacked controls) were collected, and after cDNA synthesis and PCR amplification, the resulting sample was hybridized to dot-blots spotted with genes, including some previously reported to be induced upon pathogen attack. Transcripts of several genes, (e.g., PR1a, ...Continue Reading

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Citations

Jul 10, 2007·Plant Molecular Biology·Michael Krogh JensenDavid B Collinge
Feb 18, 2011·Molecular Plant-microbe Interactions : MPMI·Matthew J MoscouRoger P Wise
May 8, 2007·Annual Review of Phytopathology·Roger P WiseSteven A Whitham
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Mar 21, 2006·Journal of Plant Physiology·Ruth EichmannRalph Hückelhoven
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Mar 31, 2005·FEMS Microbiology Letters·Ralph Hückelhoven
Nov 22, 2016·The Plant Journal : for Cell and Molecular Biology·Rémi PeyraudDominique Roby
Mar 24, 2007·Molecular Plant-microbe Interactions : MPMI·Torben GjettingMichael F Lyngkjaer
Jun 20, 2018·Proteomics·Prathap Reddy KallamadiSujatha Mulpuri
Dec 27, 2005·Journal of Experimental Botany·Luis A J MurElena Prats

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