Differential gene expression in mouse sclera during ocular development

Investigative Ophthalmology & Visual Science
Jie ZhouTerri L Young

Abstract

Ocular development involves changes in extracellular matrix components of the scleral wall as it expands. This study was conducted to determine scleral gene expression profiles during mouse ocular development to identify genes involved in normal scleral growth. Sample sets of pooled sclerae of 3- and 8-week-old mice were microdissected, and total RNA was isolated. After reverse transcription, the cDNA was in vitro transcribed to produce biotin-labeled cRNA. The purified biotin-labeled cRNA samples were hybridized to microarray chips (GeneChip Mouse Genome 430 2.0; Affymetrix, Santa Clara, CA). Gene transcript expression profiles were determined, and eight differentially expressed genes between the two age groups underwent further confirmation by real-time PCR analysis. Differential regulation of 4884 gene transcripts in mouse sclera with less than 5% false-discovery rate (FDR) was identified. The top 1000 with the lowest FDR among the 4884 probe sets were filtered for threefold changes between the two age groups, and 718 gene transcripts were identified. Among these 718 gene transcripts, 210 were upregulated and 508 downregulated in adult relative to juvenile mouse sclera. TGF-beta1 and several collagen genes were significantly...Continue Reading

Citations

Aug 19, 2007·Nucleic Acids Research·Stefano CalzaYudi Pawitan
Dec 24, 2008·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Terri L Young
Feb 14, 2013·Investigative Ophthalmology & Visual Science·Cathy NguyenHarry A Quigley
May 17, 2014·Current Eye Research·Michaël J A GirardNicholas G Strouthidis
Nov 13, 2013·Current Eye Research·Ishfaq AhmedKhurshid I Andrabi
Mar 5, 2013·Progress in Retinal and Eye Research·Denis WakefieldPeter McCluskey
Sep 11, 2013·Experimental Eye Research·Terri L YoungRavikanth Metlapally
Jul 1, 2014·Experimental Eye Research·Simona L SchlerethLudwig M Heindl
Oct 17, 2019·Taiwan Journal of Ophthalmology·Shabhat RasoolKhursheed Iqbal Andrabi

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