Differential gene expression in SV40-mediated immortalization of human fibroblasts

Journal of Cellular Physiology
J R PardinasH L Ozer

Abstract

Normal human diploid fibroblasts (HF) have a limited life span, undergo senescence, and rarely, if ever, spontaneously immortalize in culture. Introduction of the gene for T antigen encoded by the DNA virus SV40 extends the life span of HF and increases the frequency of immortalization; however, immortalization requires both T-dependent and T-independent functions. We previously generated independent SV40-transformed non-immortal (pre-immortal) HF cell lines from which we then obtained immortal sublines as part of a multifaceted approach to identify functions responsible for immortalization. In this study we undertook a search for cellular mRNAs which are differentially expressed upon immortalization. A lambda cDNA library was prepared from a pre-immortal SV40-transformed HF (HF-C). We screened the library with a subtracted probe enriched for sequences present in HF-C and reduced in immortal AR5 cells. A more limited screen was also employed for sequences overexpressed in AR5 using a different strategy. Alterations in the level of mRNAs in AR5 encoding functions relevant to signal transduction pathways were identified; however, most cDNAs encoded novel sequences. In an effort to clarify which of the altered mRNAs are most relev...Continue Reading

References

Nov 30, 1992·Biochemical and Biophysical Research Communications·S Imai, T Takano
Mar 1, 1992·Cancer Genetics and Cytogenetics·F A Ray, P M Kraemer
Aug 19, 1992·International Journal of Cancer. Journal International Du Cancer·F HoffschirB Dutrillaux
Nov 11, 1992·International Journal of Cancer. Journal International Du Cancer·A BravardB Dutrillaux
May 1, 1992·Molecular and Cellular Biology·K Hubbard-SmithH L Ozer
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y NingO M Pereira-Smith
Feb 11, 1990·Nucleic Acids Research·J H KimS M Byun
Mar 1, 1987·Mechanisms of Ageing and Development·B M Stanulis-Praeger
Aug 1, 1987·Molecular and Cellular Biology·D S NeufeldH L Ozer
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H MeunierW Vale
May 1, 1987·Molecular and Cellular Biology·M B SmallJ M Bishop
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·O M Pereira-Smith, J R Smith
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S V SuggsK Itakura
Jun 1, 1980·European Journal of Biochemistry·C Auffray, F Rougeon
Sep 2, 1983·Science·O M Pereira-Smith, J R Smith
Mar 1, 1994·Experimental Cell Research·I Rubelj, O M Pereira-Smith
Feb 1, 1995·Molecular and Cellular Biology·P BellostaC Basilico
Feb 15, 1994·Biochemical and Biophysical Research Communications·K EtoT Kadowaki
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·A K SandhuR S Athwal
Jul 1, 1993·Physiological Reviews·V J Cristofalo, R J Pignolo
Feb 23, 1996·Cell·J Campisi
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·S P Zamora-LeónJ C Vera
Jan 1, 1996·Experimental Gerontology·H L OzerP C Patsalis
Sep 1, 1996·Journal of Cellular Physiology·M B SmallK A Sethi
May 1, 1996·Trends in Biochemical Sciences·I G Wool

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.