Differential Gene Expression Patterns of Yersinia pestis and Yersinia pseudotuberculosis during Infection and Biofilm Formation in the Flea Digestive Tract

MSystems
Iman ChouikhaB Joseph Hinnebusch

Abstract

Yersinia pestis, the etiologic agent of plague, emerged as a fleaborne pathogen only within the last 6,000 years. Just five simple genetic changes in the Yersinia pseudotuberculosis progenitor, which served to eliminate toxicity to fleas and to enhance survival and biofilm formation in the flea digestive tract, were key to the transition to the arthropodborne transmission route. To gain a deeper understanding of the genetic basis for the development of a transmissible biofilm infection in the flea foregut, we evaluated additional gene differences and performed in vivo transcriptional profiling of Y. pestis, a Y. pseudotuberculosis wild-type strain (unable to form biofilm in the flea foregut), and a Y. pseudotuberculosis mutant strain (able to produce foregut-blocking biofilm in fleas) recovered from fleas 1 day and 14 days after an infectious blood meal. Surprisingly, the Y. pseudotuberculosis mutations that increased c-di-GMP levels and enabled biofilm development in the flea did not change the expression levels of the hms genes responsible for the synthesis and export of the extracellular polysaccharide matrix required for mature biofilm formation. The Y. pseudotuberculosis mutant uniquely expressed much higher levels of Yers...Continue Reading

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Citations

Jul 17, 2020·Microbiology and Molecular Biology Reviews : MMBR·Jessica L IronsDiana M Downs
Feb 7, 2021·Biomolecules·B Joseph HinnebuschDavid M Bland
Oct 31, 2021·Proceedings of the National Academy of Sciences of the United States of America·Sarah L PriceMatthew B Lawrenz
Dec 9, 2021·Archives of Microbiology·Sébastien Bontemps-GalloFlorent Sebbane

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Datasets Mentioned

BETA
GSE119243

Methods Mentioned

BETA
PCA
PCR
electrophoresis

Software Mentioned

GeneSpring
GCOS
Partek
GeneSpring GX
Partek Genomics Suite
PSAT
Expression Console
Affymetrix GeneChip

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