Apr 25, 2015

Differential Gene Expression Profile in the Rat Caudal Vestibular Nucleus is Associated with Individual Differences in Motion Sickness Susceptibility

PloS One
Jun-Qin WangYi-Ling Cai

Abstract

To identify differentially expressed genes associated with motion sickness (MS) susceptibility in the rat caudal vestibular nucleus. We identified MS susceptible (MSS) and insusceptible (inMSS) rats by quantifying rotation-induced MS symptoms: defecation and spontaneous locomotion activity. Microarray analysis was used to screen differentially expressed genes in the caudal vestibular nucleus (CVN) after rotation. Plasma stress hormones were identified by radioimmunoassay. Candidate genes were selected by bioinformatics analysis and the microarray results were verified by real-time quantitative-PCR (RT-qPCR) methods. By using Elvax implantation, receptor antagonists or recombinant adenovirus targeting the candidate genes were applied to the CVN to evaluate their contribution to MS susceptibility variability. Validity of gene expression manipulation was verified by RT-qPCR and western blot analysis. A total of 304 transcripts were differentially expressed in the MSS group compared with the inMSS group. RT-qPCR analysis verified the expression pattern of candidate genes, including nicotinic cholinergic receptor (nAchR) α3 subunit, 5-hydroxytryptamine receptor 4 (5-HT4R), tachykinin neurokinin-1 (NK1R), γ-aminobutyric acid A recept...Continue Reading

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Mentioned in this Paper

Examination of Reflexes
Serum Hormone Levels (Lab Test)
Neuro-Oncological Ventral Antigen 2
Real-Time Polymerase Chain Reaction
HTR4 gene
TACR1
Urination
Nucleus Solitarius
Biochemical Pathway
Microarray Analysis

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